荧光定量PCR检测慢性乙肝患者外周血单个核细胞乙肝病毒 DNA

目的探讨定量检测慢性乙肝患者外周血单个核细胞(PBMC)乙型肝炎病毒DNA(HBV-DNA)的临床意义.方法采用荧光定量PCR检测PBMC及血清中HBV-DNA含量.结果 49例慢性乙肝患者PBMC及血清中HBV-DNA阳性率分别为44.9%(22/49),51.0%(25/49),两者阳性率无统计学意义的差异(P＞0.05),具有一致性;血清HBV-DNA高水平组PBMC的HBV-DNA含量与低水平组比较,两者有统计学意义差异(P＜0.01);血清HBV- DNA高水平组PBMC的HBV-DNA阳性率(100%)与血清低水平组PBMC的HBV-DNA阳性率(42.9%)比较,两者有统计学意义的差异(P＜0.05);在24例血清HBV-DNA阴性患者中,发现5例PBMC HBV-DNA阳性.结论 PBMC的HBV-DNA检测可反映病毒在体内的复制程度,是对慢乙肝患者血清HBV-DNA检测有意义的补充,有助于临床上对慢乙肝患者血清病毒复制状态的了解及指导治疗用药.

Detection of HBV DNA in Peripheral Blood Mononuclear Cells with Fluorescent Quantitative PCR in Patients with Chronic Hepatitis B

Objective To study the clinical significance of quantitative detection of HBV DNA in peripheral blood mononuclear cells (PBMC) of the patients with chronic hepatitis B. Methods HBV-DNA in PBMC and serum of the patients with chronic hepatitis B was detected by fluorescent quantitative PCR (FQ-PCR) technology. Results In 49 patients with chronic hepatitis B, the positive rate of HBV-DNA in PBMC and serum was 44 9%(22/49), 51 0%(25/49) respectively, which had not significant significance (P>0 05). Between the patients with high level of serum HBV-DNA and the ones with low level of serum HBV-DNA, both the quantity and positive rate of HBV-DNA in PBMC had a significant difference (P<0 01,P<0 05). Conclusion The results suggested that the detection of HBV-DNA in PBMC can play a complementary role for monitoring the state of HBV infection and replication.