流式细胞分析术检测脱石蜡标本估计膀胱肿瘤的预后

将获得随访结果的154例膀胱癌的石蜡标本制成脱石蜡细胞核悬液,进行流式细胞分析术(FCM)检测。随访时间最短6个月,最长11年,发现DNA倍体类型,DNA指数和超二倍体细胞比值与膀胱肿瘤预后和复发有明显的关系。二倍体肿瘤的复发率为16.7％,死亡率为11.1％,而异倍体肿瘤复发率为75.6％,死亡率为64.6％,二倍体肿瘤患者生存率明显高于异倍体肿瘤患者(P<0.001),DNA指数大于1.5的异倍体肿瘤和超二倍体细胞比值大于20％的二倍体肿瘤复发率明显增高(P<0.01)。

THE PROGNOSTIC EVALUATION OF BLADDER TUMOR BY FLOW CYTOMETRIC ANALYSIS OF DEPARAFFINIZED SPECIMENS

This is a retrospective prognostic evaluation of bladder tumor by flow cytometrio analysis of deparaffinized specimens from 154 cases of transitional cell carcinoma seen in our hospital from the year 1977 to 1982. Their initial histological sections were reviewed to reconfirm the diagnosis. Deparaffinized nuclei suspensions were prepared from the .initial diagnostic paraffin-embedded pathological tissues according to the method of Hedley and associates. They had been foliowod-up for a period of 6 months to 11 years. Eesults showed that 8 of 72 oases(11.1%) with diploid tumor and 53 of 82 oases (64.6 % ) with heteroploid tumor died of the tumor. The recurrence rates in patients with diploid and heteroploid tumor were 16.6% (12/72) and 75.6% (62/82) respectively, indicating that DNA ploidy pattern was highly correlated with prognosis and recurrence of bladder tumors. Our data also revealed that tumors comprised of cells with DNA indices above 1.5 and those with greater than 20% hyperdiploidio cells had a very high recurrence rate. These results suggest that a combination of information on DNA ploidy pattern, DNA index and the incidence of hyperdiploidic cells would contribute to a more accurate prognosis of patients with bladder tumor.