Significance of Ara h 2 in clinical reactivity and effect of cooking methods on allergenicity |
| |
| Authors: | Jihyun Kim Jin Young Lee Youngshin Han Kangmo Ahn |
| |
| Affiliation: | 1. Department of Pediatrics, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Korea;2. Environmental Health Center for Atopic Diseases, Samsung Medical Center, Seoul, Korea;1. Department of Paediatrics, Prince of Wales Hospital, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China;2. Allergy and Immune Disorders, Murdoch Children''s Research Institute, Melbourne, Australia;3. Department of Paediatrics, University of Melbourne, Melbourne, Australia;4. Department of Allergy and Immunology, The Royal Children''s Hospital, Melbourne, Australia;1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, China;2. School of Life Sciences and Food Engineering, Nanchang University, Nanchang 330047, China;3. Sino-German Joint Research Institute, Nanchang University, Nanchang 330047, China;1. Division of Allergy and Immunology, Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Md;2. Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Md;3. Division of Allergy and Clinical Immunology, Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Md;4. Division of Allergy and Clinical Immunology, Department of Medicine and Pathology, Johns Hopkins University School of Medicine, Baltimore, Md;1. Department of Pediatric Pneumology and Immunology, Charité Universitätsmedizin Berlin, Berlin, Germany;2. Division of Allergy and Clinical Immunology, Department of Pediatrics, Federal University of São Paulo, São Paulo, Brazil;3. VBC Genomics, Vienna, Austria;4. Institute of Food Research, Norwich, United Kingdom;5. Pediatric Allergology and Pneumology, German Red Cross Hospital Westend, Berlin, Germany;1. Department of Dermatology and Allergology, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands;2. Department of Experimental Immunology, Amsterdam University Medical Centers, Location AMC, Amsterdam, The Netherlands;3. Department of Clinical Epidemiology, Biostatistics and Bioinformatics, Amsterdam University Medical Center, Amsterdam, The Netherlands;4. Ambulatorio di Allergologia, Clinica San Carlo, Paderno Dugnano, Italy;5. Clinical Epidemiology Unit, Preventive Medicine Department, Hospital Clinico San Carlos, IdISSC, Madrid, Spain;6. Medical Faculty and Faculty Hospital Pilsen, Charles University, Pilsen, Czech Republic;7. Allergy Division, Chest Disease Department, Strasbourg University Hospital, University of Strasbourg, Strasbourg, France;8. Landspitali University Hospital, Faculty of Medicine, University of Iceland, Reykjavik, Iceland;9. Medical Faculty, Vilnius University, Vilnius, Lithuania;10. Allergy Unit, Department of Dermatology, University Hospital of Zürich, Zürich, Switzerland;11. Medical University of Vienna, Department of Pathophysiology and Allergy Research, Vienna, Austria;12. Department of Immunology, Rheumatology and Allergy, Faculty of Medicine, Medical University of ?ód?, ?ód?, Poland;13. Clinical Centre of Allergology, Medical University of Sofia, Sofia, Bulgaria;14. Thermo Fisher Scientific, Uppsala, Sweden;15. Allergy Department, 2nd Pediatric Clinic, University of Athens, Athens, Greece;p. Division of Infection, Immunity & Respiratory Medicine, Manchester Institute of Biotechnology, University of Manchester, Manchester, United Kingdom;q. University Hospital Sv. Ivan Rilski, Sofia, Bulgaria;r. Allergy Division, Chest Disease Department, Strasbourg University Hospital, Strasbourg, France;s. Allergy Department, Hospital Clinico San Carlos, IdISSC, Madrid, Spain;t. Institute of Immunity and Transplantation, University College London, London, United Kingdom;u. Department of Nutritional Sciences and Dietetics, International Hellenic University, Thessaloniki, Greece;v. Paul-Ehrlich-Institut, Federal Institute for Vaccines and Biomedicines, Langen, Germany;w. Division of Internal Medicine and Dermatology, University Medical Center Utrecht, Utrecht University, Utrecht, The Netherlands;x. Faculty of Medicine, University of Zürich, Zürich, Switzerland;y. Clinic for Dermatology and Allergology, Kantonsspital St Gallen, St Gallen, Switzerland;z. Allergy Department, Hospital Clinico San Carlos, IdISSC, ARADyAL, Madrid, Spain;11. Department of Otorhinolaryngology, Amsterdam University Medical Centers, Location AMC, Amsterdam, The Netherlands |
| |
| Abstract: | BackgroundThe prevalence and clinical severity of peanut allergy vary between Western countries and Asia. It has been suggested that cooking methods are responsible for this discrepancy.ObjectivesTo evaluate the specific IgE responses to major peanut allergens in peanut allergic Korean children and to examine the influence of different cooking methods on peanut proteins.MethodsRaw peanut protein extracts were immunolabeled with serum samples from 42 children with a level of peanut specific IgE of 15 kUA/L or higher to detect specific binding to Ara h 1, Ara h 2, and Ara h 3. Clinical severity scores were assessed on a scale of 0 to 5. Protein extracts from boiled, roasted, fried, and pickled peanuts were analyzed by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and immunoblotting with pooled serum samples from 7 patients.ResultsMost patients' serum samples reacted with Ara h 1 (76.2%) and Ara h 3 (78.6%) from raw peanuts, whereas only 53.0% of patients had specific IgE against Ara h 2. IgE binding to Ara h 2 was more prevalent in patients with more severe reaction than in those with mild reactions. IgE binding to Ara h 2 was increased by roasting, but there was significantly less IgE binding after vinegar treatment.ConclusionOur results suggest that Ara h 2 is an important allergen to predict clinical symptoms but less prevalent in Korean children than in Western children. This finding may be attributed in part to different cooking methods and dietary habits among regions. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
