| 镁离子抗氧化作用的实验研究 |
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| 引用本文： | 孙设宗,毛达勇,卢方安,张红梅.镁离子抗氧化作用的实验研究[J].郧阳医学院学报,2005,24(5):260-262. |
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| 作者姓名： | 孙设宗 毛达勇 卢方安 张红梅 |
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| 作者单位： | 郧阳医学院生化与免疫学综合实验室 湖北十堰442000
(孙设宗，卢方安)，郧阳医学院附属太和医院检验部 湖北十堰442000
(毛达勇)，郧阳医学院生化与免疫学综合实验室 湖北十堰442000(张红梅) |
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| 基金项目： | 2005年度郧阳医学院中青年基金资助计划项目(2005ZQY02) |
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| 摘 要： | 目的:探讨镁离子抗氧化作用的机制。方法:3月龄昆明种小鼠50只,随机分为正常对照组、氧化对照组、不同浓度镁保护组,饲养10 d后,腹腔注射0.15%CC l4致小鼠肝损伤,将氧化对照组、不同浓度镁保护组肝匀浆中加入FeSO4和H2O2,诱导自由基生成,观察镁离子在体内、外的抗氧化作用。结果:镁离子能显著降低小鼠血清中ALT和AST活性,以及肝细胞中脂质过氧化物MDA的含量;体外实验镁离子对MDA生成有显著的抑制作用。结论:镁离子能抑制自由基的生成,促进自由基的清除。
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| 关 键 词： | 镁离子 抗氧化 小鼠 自由基 |
| 文章编号： | 1006-9674（2005）05-0260-03 |
| 收稿时间： | 2005-04-05 |
| 修稿时间： | 2005年4月5日 |
The research of anti-oxidative mechanism of Mg2+ |
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| SUN She - zong, MAO Da - yong, LU Fang- an, et al.The research of anti-oxidative mechanism of Mg2+[J].Journal of Yunyang Medical College,2005,24(5):260-262. |
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| Authors: | SUN She - zong MAO Da - yong LU Fang- an |
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| Abstract: | Objective To explore the anti-oxidative mechanism of Mg~(2 ).Methods Fifty Kunming mice in 3 months were randomly divided into control group,oxidation group and different level of Mg~(2 ) groups.After 10 days of breeding,all mice were injected intraperitoneally with 0.15% CCl_4,hepatic injury was induced.To induce the production of free radical,FeSO_4 and H_2O_2 were added to the hepatic homogenate of oxidation group and different level of Mg~(2 ) group.The anti-oxidation of Mg~(2 ) both in vivo and vitro was measured.Results There was a significant reduction in serum ALT and AST activity and MDA;the production of MDA was inhibited markedly by Mg~(2 ) in vitro.Conclusion Mg~(2 ) can inhibit the production of free radical and accelerate the clearance of free radical. |
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| Keywords: | magnesium anti-oxidation mouse free radical |
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