| 煤烟颗粒提取物对大鼠肺细胞的氧化 性损伤作用 |
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| 引用本文： | 李云晖,浦跃朴,尹立红,仲伟鉴,张荣泉.煤烟颗粒提取物对大鼠肺细胞的氧化 性损伤作用[J].中国公共卫生,2001,17(10):901-902. |
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| 作者姓名： | 李云晖 浦跃朴 尹立红 仲伟鉴 张荣泉 |
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| 作者单位： | 1. 东南大学公共卫生学院,
2. 上海市疾病预防控制中心 |
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| 基金项目： | 国家自然科学基金课题(39100030) |
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| 摘 要： | 目的：通过加入抗氧化剂N－乙酰半胱氨酸，作为实验干预手段。方法：观察煤烟对大鼠肺Ⅱ型细胞生长和DNA交联形成作用的影响。结果：与对照组相比，分别加入10、30mmol/L N－N乙酰半胱氨酸后的实验组细胞毒性有所下降， 从而间接地证实了氧化性损伤的存在；同时，N－乙酰半胱氨酸还可以降低煤烟经细胞染毒所致的DNA交联作用 。结论：煤烟可能是经过细胞代谢活化后产生的某些代谢产物而导致细胞的氧化性损伤。
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| 关 键 词： | 煤烟 自由基 肺细胞 抗氧化剂 氧化性损伤 环境污染 |
| 文章编号： | 1001-0580(2001)10-0901-02 |
| 修稿时间： | 2000年1月10日 |
Oxidative Stress Damages Induced by Soot on Rat Pulmonary Cells |
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| LI Yun-hui,et al..Oxidative Stress Damages Induced by Soot on Rat Pulmonary Cells[J].Chinese Journal of Public Health,2001,17(10):901-902. |
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| Authors: | LI Yun-hui |
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| Institution: | LI Yun-hui,et al.College of Public Health,Southeast University |
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| Abstract: | Objective To study the oxidative stress damages induced by soot on rat pulmonary cells.Methods The rat pulmonary cells were pretreated with anti-oxidative N-ace tylcysteine (NAC) for 30 minutes , then they were exposed to the soot extracts for 24 hours. MTT assays and ethidium bromide fluorescence assays(EFA) were applied to detect the cytotoxicity and DNA cross-links.Results The results showed that NAC could reduce the cytotoxicity and DNA cross-links of rat pulmonary cell caused by soot.Conclusion It was suggested that the soot can lead to oxidative stress on cells. |
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| Keywords: | soot rat pulmonary cell oxidative stress cytotoxicity DNA damage |
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