Ultrastructure of endometrial epithelial cells in a three-dimensional cell culture system for human implantation studies |
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Authors: | U. Bentin-Ley S. Lindenberg T. Horn J. Falck Larsen |
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Affiliation: | (1) Department of Obstetrics-Gynecology, Herlev Hospital, University of Copenhagen, DK 2730 Herlev, Denmark;(2) Department of Pathology, Herlev Hospital, University of Copenhagen, DK 2730 Herlev, Denmark;(3) Ciconia Fertility Clinic, Frydendahlsvej 5, DK 1809 Frederiksberg C, Denmark;(4) Department of Obstetrics and Gynecology G113 LT, Herlev University Hospital, Herlev Ringvej 75, DK-2730 Herlev, Denmark |
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Abstract: | Purpose A three-dimensional cell culture system imitating normal uterine endometrium has previously been established. To what degree do cultured epithelial cells retain their morphological characteristics as compared to in vivomaterial obtained simultaneously from the same tissue donor.Results We found a high degree of similarity between the in vivoand in vitrosituations. The present culture system furthermore imitates the day-to-day morphology of the cycle.Conclusions This indicates, that a correct timing of the biopsy tissue is important for future human implantation studies.Presented at the IXth World Congress on In Vitro Fertilization and Alternate Assisted Reproduction, April 3–7, 1995, Vienna, Austria. |
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Keywords: | cells cultured endometrium human in vitro ultrastructure, implantation |
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