卵巢上皮癌组织及腹腔液CyclinE mRNA的表达及意义

目的 :研究 Cyclin E m RNA与卵巢上皮癌发生、发展的关系 ;探讨检测卵巢上皮性肿瘤腹腔液中 Cyclin E m RNA的表达 ,在诊断卵巢上皮癌中的价值。方法 :用 RT- PCR法检测 39例卵巢上皮癌组织及腹腔液中 Cyclin E m RNA的表达 ,腹腔液常规作细胞学检查 ;同时检测 2 6例良性卵巢上皮性肿瘤及 1 6例正常卵巢组织作对照。结果 :Cyclin Em RNA分别在正常卵巢、良性及恶性卵巢上皮性肿瘤组织中及腹腔液中的阳性率 ,差别均无显著性 ( P>0 .0 5 ) ,组织中及腹腔液中的 Cyclin E m RNA阳性率均随正常卵巢、良性及恶性卵巢上皮性肿瘤进展而增加表达 ( P<0 .0 5 ) ,并均与卵巢上皮癌的临床分期、组织分化、淋巴结转移及腹水的有无有关 ( P<0 .0 5 ) ,与病理类型无关 ( P>0 .0 5 ) ;腹腔液中 Cyclin Em RNA的阳性率与细胞学差异无显著性 ( P>0 .0 5 ) ,并且两者无相关性。结论 :Cyclin Em RNA的高表达与卵巢上皮癌的发生、发展及恶性程度密切相关 ,可作为预后不良的重要指标。检测卵巢上皮性肿瘤腹腔液中 Cyclin E m RNA的表达可鉴别肿瘤的性质 ,判断预后

Detection of CyclinE mRNA in epithelial ovarian neoplasms tissue and ascites using RT-PCR

Objective:To study the relationship between the expression of CyclinE mRNA and the occurrence and development of epithelial ovarian tumors and to investigate the value of CyclinE mRNA in diagnosis of epithelial ovarian neoplasms.Method:The tissues and ascites were collected during laparotdomy in 39 patients with epithelial ovarian neoplasms,26 patients with benign epithelial ovarian tumor and 16 cases normal ovarian.Tissues CyclinE mRNA by was tested RT-PCR and ascited by the conventional cytological method.Results:The CyclinE mRNA positive rate ascites in normal ovarian tissues,benign and malignant epithelial ovarian tumors showed no obvious difference (P>0.05), the CyclinE mRNA positive rate increased with development of ovarian tumors(P<0.05),and was associated with advanced stage,low grade,presence of ascites and lymph node metastasis(P<0.05) but was not associated with histopathological type(P>0.05).There were no significant difference in the CyclinE mRNA positive rate between malignant ascites and cytologic examination(P>0.05),and the CyclinE mRNA positive expression in malignant ascites was not correlated with cytologic examination(τ=0.178, P>0.05).Conclusions:(1)High expression of CyclinE mRNA was correlated to the origination,progression and malignancy of epithelial ovarian carcinoma and might bea poor prognostic factor for patients with epithelial ovarian carcinoma.(2)Detection of CyclinE mRNA in epithelial ovarian tumors ascites by RT-PCR was the efficient method for the determination of malignancy and prognosis.If cytologic examination in ascites is investigated simultaneously ,the sensitivity could be further enhanced.