持续低温灌注对大鼠移植肝脏的保护作用

目的 探讨离体供肝持续低温灌注对大鼠移植肝脏的保护作用.方法 60只Wistar大鼠随机分为3组,假手术组12只.离体供肝进行持续性冷灌注组和常规冷保存组各24只,供肝保存时间5 h.假手术组及后两组受体分别于术后24 h留取血液、肝组织,并记24 h平均胆汁流量.各组分别检测肝功能,测定胆汁中γ-谷氨酰转肽酶(GGT)活性及萄萄糖(Glu)含量.肝组织行常规病理学检查.结果 持续低温灌注组和常规冷保存组血清肝功能指标均高于假手术组(P＜0.05),而持续低温灌注组较常规冷保存组低(P＜0.05).持续低温灌注组平均每小时胆汁流量明显高于冷保存组(P＜0.05).持续低温灌注组胆汁中GGT活性和Glu含量明显低于常规冷保存组(P＜0.05).结论 离体供肝持续低温灌注对大鼠移植后肝功能有明显的保护作用.

Protective effect of continuous hypothermic perfusion on transplanted liver in rats

Objective To explore the protective effect of continuous hypothermic perfusion on donor liver. Methods Sixty Wistar rats were randomly divided into the control group (sham operation group, n＝12), donor continuous hypothermic reperfusion group (n＝24) and static cold storage group (n＝24). The donor keeping time was 5 h. The samples of blood and hepatic tissue were col-lected 24 h after liver transplantation. Meanwhile, the bile flow during the 24 h was recorded. Results Compared with the control group, liver functional parameters were higher in other two groups (P＜0.05). It was significantly lower in the continuous hypothermic reperfusion group than in the static cold storage group (P＜0.05). Meanwhile, the bile flow in the continuous hypothermic reperfusion group was more than that in the static cold storage group (P＜0.05). While γ-GGT and the Glucose(Glu) in the bile of the continuous hypothermic reperfusion group were lower than those in the static cold storage group (P＜0.05). Conclusions Continuous hypothermic perusion of donor liver during the keeping time has protective effect on the liver after transplantation.