PAX1基因启动子甲基化检测对宫颈癌及 宫颈癌前病变筛查的价值研究

目的 探讨配对盒基因家族PAX1基因启动子异常甲基化在宫颈癌和癌前病变早期诊断中的价值.方法 应用甲基化特异性聚合酶链反应（MSP）、高分辨率溶解（HRM）技术,对人乳头瘤病毒（HPV）感染的正常宫颈脱落细胞、宫颈炎脱落细胞、CIN Ⅰ脱落细胞、CINⅡ～Ⅲ脱落细胞及宫颈癌脱落细胞中的PAX1基因启动子进行甲基化检测,比较两种方法对不同级别宫颈病变的敏感度和特异度.结果 两种方法宫颈癌脱落细胞组与正常＋宫颈炎脱落细胞组以及CINI,CINII～III宫颈脱落细胞组相比,差异均有统计学意义（P＜0.05）.MSP法和HRM法检测PAX1基因启动子甲基化筛查宫颈癌的敏感度、特异度、阳性预测值和阴性预测值分别为54.55％,89.66％,66.67％,83.87％和72.73％,87.93％,69.57％,89.47％.结论 PAX1基因启动子甲基化对于宫颈癌临床诊断具有重要价值,HRM技术应用前景广阔.

The Role of DNA Hypermethylation of PAX1 in Early Diagnosis of Cervical Cancer and Precursor Lesions

Objective In order to explore the value of aberrant methylation of Paired box gene family PAX1 in the early diagnosis of cervical neoplasm and precancerous lesions. Methods By examining the hypermethylation of PAX1 in the HPV-infected exfoliated cells of normal, cervicitis, CINI, CINII -III and cervical cancer with MSP I methylation-specific polymerase chain reaction） and HRM （ high-resolution melting） ,comparing the differences of sensitivity and specificity to various stages of cervical lesion with different methods. Results In both of two methods, comparing with the hypermethylation of the exfoliated cells of normal, cervicitis, CINI, CINII -III, the differences in cer- Vical cancer exfoliated cells were statistically significant I P 〈 0.05 }. The method of MSP： The sensitivity, specificity, PPV and NPV to cervical Cancer were 54.55% ,89.66%, 66.67% and 83.87%. The method of HRM：The sensitivity, specificity, PPV and NPV to cervical cancer were 72.73% ,87.93% ,69.57% and 89.47%. Conclution The detection of the methylation of PAX1 is important for clinical diagnosis of eervical cancer. There is a bright future for HRM.