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| 树鼩、熊猴感染人乙型肝炎病毒肝细胞内感染指征的研究 | |
| 引用本文： | 王树声,苏建家,冯百芳,李媛,张涛,覃柳亮,黄果勇,高建恩,葛宪民,李河民.树鼩、熊猴感染人乙型肝炎病毒肝细胞内感染指征的研究[J].中华实验和临床病毒学杂志,2003,17(3):244-246,T003. |
| 作者姓名： | 王树声 苏建家 冯百芳 李媛 张涛 覃柳亮 黄果勇 高建恩 葛宪民 李河民 |
| 作者单位： | 1. 530021,南宁,广西壮族自治区疾病预防控制中心 2. 广西肿瘤研究所 3. 北京大学肝病研究所 4. 中国药品生物制品检定所 |
| 基金项目： | 国家“九五”科技攻关课题 (96 90 6 3 0 10 ) |
| 摘 要： | 目的 对树鼩、熊猴感染人乙型肝炎病毒(HHBV)后对肝细胞病变进行动态观察。方法 10只成年树鼩，28只熊猴接种含人乙型肝炎病毒(HBV)血清后，定期肝活检，采用HE染色、免疫组化、原位杂交对实验动物肝组织进行研究分析。结果 80％树鼩感染HHBV后，通过免疫组化在肝组织内可找到乙型肝炎病毒表面抗原(HBsAg)，50％通过原位杂交可检测到HBV DNA。有25％熊猴的肝组织内可检测到HBsAg，但信号较弱，而肝组织内未发现HBV DNA。结论 树鼩感染HHBV后，肝细胞内出现病理改变，适用于对人乙型肝炎的研有． |
| 关 键 词： | 乙型肝炎病毒 肝细胞 感染指征 树鼩 熊猴 动物模型 免疫组织化学 |
Serial pathologic changes in livers of tree shrwes and macaca assamensises infected with human hepatitis B virus |
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| WANG Shu sheng ,SU Jian jia,FENG Bai fang,LI Yuan,ZHANG Tao,QIN Liu liang,HUANG Guo yong,GAO Jian en,GE Xian min,LI He min Guangxi Center for Diseases Prevenfion and Control,Nanning ,China.Serial pathologic changes in livers of tree shrwes and macaca assamensises infected with human hepatitis B virus[J].Chinese Journal of Experimental and Clinical Virology,2003,17(3):244-246,T003. | |
| Authors: | WANG Shu sheng SU Jian jia FENG Bai fang LI Yuan ZHANG Tao QIN Liu liang HUANG Guo yong GAO Jian en GE Xian min LI He min Guangxi Center for Diseases Prevenfion and Control Nanning China |
| Institution: | WANG Shu sheng *,SU Jian jia,FENG Bai fang,LI Yuan,ZHANG Tao,QIN Liu liang,HUANG Guo yong,GAO Jian en,GE Xian min,LI He min * Guangxi Center for Diseases Prevenfion and Control,Nanning 530021,China |
| Abstract: | Objective To serially observe the pathologic changes in livers of tree shrews and macaca assamensises infected with HHBV Methods 10 adult tree shrews and 28 macaca assamensises were inoculated with HBV rich human sera The liver of the animals were regularly biopsied The liver samples were examined histopathologically by HE staining Some samples were stained for HBsAg by immunohistochemistry (IH), and HBV DNA by in situ hybridization (ISH) Results HBsAg in 80% of tree shrews infected with HHBV can be detected by IH, HBV DNA in 50% of those can be found by ISH. The positive rates of HBsAg in macaca assamensises ' livers were 25% by IH, none HBV DNA was detected Conclusion The tree shrew model seems to be applicable for the research of human hepatitis B |
| Keywords: | Hepatitis B Animals labratory Tupalidae Macaca |
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