大鼠坐骨神经切断后P-Erk1/2在相应背根神经节中的表达变化 |
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引用本文: | 朱春雷,徐明珠,吴广智,崔树森. 大鼠坐骨神经切断后P-Erk1/2在相应背根神经节中的表达变化[J]. 中国实验诊断学, 2009, 13(3): 345-347 |
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作者姓名: | 朱春雷 徐明珠 吴广智 崔树森 |
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作者单位: | 吉林大学中日联谊医院,手外科,吉林,长春130033 |
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摘 要: | 目的研究坐骨神经切断后P-ERK1/2在成年大鼠相应背根神经节中的表达变化。方法选取健康成年雄性Wister大鼠80只,随机分为假手术组和坐骨神经切断组,分别于术后1、2、4、6、8周五个时相处死后取患侧L4-L6背根神经节,应用Western Blot和免疫组织化学技术检测P-ERK1/2在背根神经节中的表达变化.将两组所得数值进行统计学分析。结果假手术组中背根神经节中P-EILK1/2,有少量表达并维持一定的水平.坐骨神经切断组,1周时P-ERK1/2表达已经增加并达高峰,2周后逐渐下调,8周恢复至对照组水平。结论坐骨神经切断后可通过激活MAPK/ERK1/2信号通路的方式,对背根神经节中感觉神经元的凋亡进行调控。
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关 键 词: | 胞外信号调节激酶 背根神经节 神经损伤 |
Change and expression of phospho-Erk1/2 in rat DRG after sciatic nerve transection |
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Affiliation: | ZHU Chun-lei, XU Ming-zhu, WU Guang-zhi, et al. ( China-Japan Union Hospital of Jilin University , Changchun 130033, China) |
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Abstract: | Objective To investigate the expression changes of phospho-Erk1/2 in rat Dorsal root ganglion after sciatic nerve transaction.Methods Adult male waster rats were randomly divided into the normal control pseudo-group and the experimental operatlon-group.In the operation-group,the fight sciatic nerve was transected and survived for 1,2,4,6 and 8 weeks after operation.The changes in the expression of p-Erk1/2 in L4-5 DR(; were detected by western blot and immunohistochemical methods.Results In Pseudo-operation group p-ERK1/2 only has a small amount of expression and maintain a certain level in dorsal root ganglion.In the experimental operation-group, p-ERK1/2 expression has increased and reached the peak in 1 week after sciatic nerve transaction.The p-ERK1/2 expression began to decrease at 2 weeks. The expression was back to the normal level at 8 weeks. Conclusion Sciatic nerve transaction is likely to, activate MAPK/ERK1/2 signaling pathway which can control apoptosis of Dorsal root ganglion. |
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Keywords: | extracellular signal regulated kirtase(ERK) dorsal mot ganglion(DRG) never injury |
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