大鼠坐骨神经切断后P-Erk1/2在相应背根神经节中的表达变化

目的研究坐骨神经切断后P-ERK1／2在成年大鼠相应背根神经节中的表达变化。方法选取健康成年雄性Wister大鼠80只，随机分为假手术组和坐骨神经切断组，分别于术后1、2、4、6、8周五个时相处死后取患侧L4-L6背根神经节，应用Western Blot和免疫组织化学技术检测P-ERK1／2在背根神经节中的表达变化．将两组所得数值进行统计学分析。结果假手术组中背根神经节中P-EILK1／2，有少量表达并维持一定的水平．坐骨神经切断组，1周时P-ERK1／2表达已经增加并达高峰，2周后逐渐下调，8周恢复至对照组水平。结论坐骨神经切断后可通过激活MAPK／ERK1／2信号通路的方式，对背根神经节中感觉神经元的凋亡进行调控。

Change and expression of phospho-Erk1/2 in rat DRG after sciatic nerve transection

Objective To investigate the expression changes of phospho-Erk1/2 in rat Dorsal root ganglion after sciatic nerve transaction.Methods Adult male waster rats were randomly divided into the normal control pseudo-group and the experimental operatlon-group.In the operation-group,the fight sciatic nerve was transected and survived for 1,2,4,6 and 8 weeks after operation.The changes in the expression of p-Erk1/2 in L4-5 DR（; were detected by western blot and immunohistochemical methods.Results In Pseudo-operation group p-ERK1/2 only has a small amount of expression and maintain a certain level in dorsal root ganglion.In the experimental operation-group, p-ERK1/2 expression has increased and reached the peak in 1 week after sciatic nerve transaction.The p-ERK1/2 expression began to decrease at 2 weeks. The expression was back to the normal level at 8 weeks. Conclusion Sciatic nerve transaction is likely to, activate MAPK/ERK1/2 signaling pathway which can control apoptosis of Dorsal root ganglion.