Increased intestinal absorption of insulin in a micellar solution: Water-in-oil-in-water insulin micelles |
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Authors: | Motoaki Shichiri Ryuzo Kawamori Yoshikazu Goriya Mikio Kikuchi Yoshimitzu Yamasaki Yukio Shigeta Hiroshi Abe |
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Affiliation: | (1) First Department of Medicine, Osaka University Medical School, Fukushima 1-1-50, Fukushima-ku, Osaka, Japan |
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Abstract: | Summary Water-in-oil-in-water (W/O/W) insulin micelles were prepared, and the possibility of insulin absorption in a micellar form was examined. In this preparation, insulin was trapped in oil droplets of oleic acid in glyceryl-α-monooleate. (1) W/O/W insulin micelles were absorbed from the ligated jejunal loop of rabbits to the order of 12.3 to 58.5% of the dose given (10 U/kg body weight) during the 3-h experimental period. (2) Alloxan diabetic rats were treated with intrajejunal administration of W/O/W insulin micelles at a dosage of either 25 or 50 U/100 g body weight, three times daily for as longs as 14 days. During treatment, a significant reduction in the daily excretion of urinary glucose was observed, concomitant with a decrease in fasting blood glucose. Quantitative estimates suggested that the effectiveness of 25 U/100 g of intrajejunal W/O/W insulin micelles was comparable to that of regular insulin at a dosage of 1 U/100 g i.m. These results would indicate that W/O/W insulin micelles, when given enterally, are more effective in lowering blood and urinary glucose levels than W/O/W insulin emulsions in which insulin was trapped in oil droplets of triglyceride. |
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Keywords: | Alloxan diabetic rat Insulin micelles Intestinal absorption of insulin Rabbit Water-in-oil-in-water micelles |
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