胞内、外Ca2+在水杨酸诱导丹参幼苗迷迭香酸生物合成过程中的作用

目的: 为探讨胞内、外钙离子是否在水杨酸诱导丹参迷迭香酸生物合成中起作用。 方法: 以生长50 d的丹参幼苗为材料,通过喷施水杨酸诱导迷迭香酸合成量增加,再利用胞外钙通道抑制剂verapamil(Vp)和LaCl₃、胞内钙调素拮抗剂trifluoperazine (TFP)和胞内钙通道上IP₃受体抑制剂LiCl处理,分别考察水杨酸、钙离子通道抑制剂/钙调素拮抗剂处理后迷迭香酸的合成量及其合成相关酶(PAL,TAT)的活性变化。 结果: 2.0 mmol·L^-1的水杨酸处理24 h后可诱导迷迭香酸合成量升高到(40.51±2.16) mg·g^-1,是对照的1.97倍,迷迭香酸合成相关酶PAL活性升高到对照的1.42倍,TAT活性升高到对照的1.29倍;Vp,LaCl₃,TFP,LiCl处理均抑制了水杨酸诱导的PAL,TAT活性的升高,从而导致了迷迭香酸合成量降低。 结论: 水杨酸诱导丹参迷迭香酸生物合成过程中,胞内、外钙离子发挥着重要的信号转导作用。

Regulation effects of intracellular and extracellular Ca2+ on biosynthesis of rosmarinic acid induced by salicylic acid in young seedlings of Salvia miltiorrhiza

Objective: To investigate the effect of intracellular and extracellular Ca²⁺ on the biosynthesis of rosmarinic acid (RA) induced by salicylic acid in young seedlings of Salvia miltiorrhiza. Method: Young seedlings of S. miltiorrhiza were used to select an optimal concentration of salicylic acid(SA), and then use the optimal concentration of SA to investigate the effects of extracellular Ca²⁺ channel inhibitors Verapamil,LaCl₃, intracelluar calmodulin antagonist TFP and intracelluar Ca²⁺ channel inhibitors LiCl on the biosynthesis of RA and related enzymes. Result: SA increased the accumulation of RA and the activities of PAL and TAT, especially the SA of 2 mmol·L^-1 after 24 h. SA improved the accumulation of RA to (40.51±2.16) mg·g^-1, which was 1.97 times than that of control, and the activities of PAL, TAT were 1.42 times and 1.29 times than those of the control. However, Vp,LaCl₃, TFP, LiCl inhibited the effects of SA evidently. Conclusion: Ca²⁺ plays a key role in the regulation of the induction process.