葛根芩连煎剂中小檗碱在犬体内药动学研究

目的:测定葛根芩连煎剂与黄连单煎剂犬血中小檗碱的含量,分析配伍后(全方)对小檗碱体内过程的影响。方法:采用高效液相色谱法,测定不同时间点犬血浆中小檗碱的含量,WinNonlin软件计算药动学参数。结果:葛根芩连煎剂及黄连单煎剂中小檗碱在犬体内药-时曲线均符合一室模型,主要药动学参数AUC(0-∞)(1·25±0·06)、(14·71±0·54)(μg·h)/ml,tmax(1·92±0·31)、(3·03±0·07)h,Cmax(0·17±0·01)、(1·79±0·03)μg/ml。结论:葛根芩连煎剂配伍后与单方比较小檗碱入血浓度及生物利用度都降低。

Pharmacokinetics of Berberine in Puerariae and Scutellariae and Coptidis Decoction in Vivo of Dog

OBJECTIVE:To assay serum berberine in Puerariae and Scutellariae and Coptidis Decoction and coptidis rhiˉzoma single decoction in dog and to analyze the effect of the compound recipe(whole recipe)on the physiological disposition of beberine.METHODS:Serum concentrations of berberine in dog were determined at different time by HPLC and the pharˉmacokinetic parameters were computed with WinNonlin software.RESULTS:The concentration-time curves of berberine in Puerariae and Scutellariae and Coptidis Decoction and coptidis rhizoma single decoction in vivo of dog conformed to one-compartment model,the main pharmacokinetic parameters of berberine in Puerariae and Scutellariae and Coptidis Decoction vs.in coptidis rhizoma single decoction were as follows,AUC (0-∞) (1.25±0.06)vs.(14.71±0.54)(μg·h)/ml,t max (1.92±0.31)vs.(3.03±0.07)h,C max (0.17±0.01)vs.(1.79±0.03)μg/ml.CONCLUSIONS:As compared with single-item recipe,the plasma concentration and bioavailability of berberine in the compound recipe of Puerariae and Scutellariae and Coptidis Decocˉtion were all lower.