| 用地高辛配基标记DNA探针进行菌落原位杂交 |
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| 引用本文： | 蒋三亮,张思仲. 用地高辛配基标记DNA探针进行菌落原位杂交[J]. 四川大学学报(医学版), 1992, 0(3) |
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| 作者姓名： | 蒋三亮 张思仲 |
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| 作者单位： | 华西医大医学遗传学研究室(蒋三亮)，华西医大医学遗传学研究室(张思仲) |
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| 摘 要： | 用地高辛配基标记DNA探针,结合酶联免疫反应检测显示信号。建立了一种简便,快速的菌落原位杂交新方法。结果证明该方法敏感、稳定,完全可取代同位素用于细菌分析和克隆筛选。
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| 关 键 词： | 地高辛配基 DNA探针 菌落原位杂交 克隆筛选 |
Colony Hybridization with Digoxingenin Labelled DNA Probe |
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| Jiang Sanliang,Zhang Sizhong. Colony Hybridization with Digoxingenin Labelled DNA Probe[J]. Journal of Sichuan University. Medical science edition, 1992, 0(3) |
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| Authors: | Jiang Sanliang Zhang Sizhong |
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| Affiliation: | Jiang Sanliang;Zhang Sizhong Department of medical Genetics |
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| Abstract: | A simple, rapid method for co-lony hybridization has been developed.TheDNA probes were labelled by digoxingenin.Hybridization signal was detected by enzyme-linked immunoassay reaction. The resultsshowed that this method is sensitive andreproducible, and it can be used for colonyhybridization instead of isotopic methods. |
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| Keywords: | Digoxingenin DNA probe Colony hybridization Colony screening |
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