DFOG对A549细胞生长及FoxM1表达的影响

目的:研究7-二氟甲氧基-5,4′-二-正辛烷氧基金雀异黄素(DFOG)抑制人肺癌细胞生长作用及机制.方法:用不同浓度DFOG处理体外培养A549细胞后,流式细胞术(FCM)检测细胞周期分布；平皿集落形成法测定细胞生长；Western blot分析FoxM1蛋白表达.结果:DFOG处理24 h导致A549细胞系G2期细...

Effects of DFOG on inhibition of growth and expression of FoxM1 in A549 cell line

Objective To investigate the effects of 7-difluoromethyl-5,4’-di-n-octylgenistein(DFOG) on the growth of human lung caner A549 cells and its mechanism.Methods A549 cells were cultured in vitro and were treated with various concentrations of DFOG.The ability of cell colony formation was detected by the colony formation on plate.The cell cycle phase distribution was determined by flow cytometry(FCM) analysis.The expression of FoxM1 was analyzed by Western blot.Results FCM analysis showed that DFOG resulted in cell cycle arrest in G2 phase.The clonogenic assay indicated that DFOG significantly inhibited the anchorage-dependent growth of A549 cells(P<0.05).Exposure of A549 cells to DFOG(2.0 μmol/L) at 6 h,12 h and 24 h,the level of FoxM1 expression decreased,in time-depentment manner.Conclusion DFOG inhibited the growth of A549 cells is associated with downregulation of FoxM1 expression.