钛种植体SLA表面HA涂层对骨髓源成骨细胞生物学特性的影响

目的:探讨表面喷砂和酸蚀(SLA)处理后羟基磷灰石(HA)涂层的钛种植体(HA-SLA-Ti),对骨髓源成骨细胞(MOOBs)生物学特性的影响。方法:应用离子束辅助沉积技术(IBAD)在SLA处理的钛种植体(SLA-Ti)表面制备HA涂层,将MOOBs分别接种于HA-SLA-Ti和SLA-Ti表面,观察其生长情况,并对2组MOOBs增殖指数、碱性磷酸酶活性、骨钙素含量以及骨桥蛋白基因(opn)mRNA相对表达量进行比较。结果:MOOBs在HA-SLA-Ti表面生长良好,其细胞增殖指数、碱性磷酸酶活性以及骨钙素含量明显高于SLA-Ti组;且opnmRNA相对表达量是SLA-Ti组的3.25倍。结论:应用IBAD技术在钛种植体SLA表面制备HA涂层,可明显促进MOOBs的增殖及其成骨表型的表达,是一种有应用前景的种植体表面处理方法。

The influence of hydroxyapatite coating on sandblasted and acid-etched surface of titanium dental implants on the biologic characteristics of marrow-origin osteoblasts

Objective:To study the effects of hydroxyapatite(HA) coating on sandblasted and acid-etched(SLA) surface of titanium dental implants(HA-SLA-Ti) on the biologic characteristics of marrow-origin osteoblasts(MOOBs).Methods:Coating of HA on the SLA surface of titanium dental implants were formed by ion beam assisted deposition (IBAD) method. MOOBs,cultured in vitro,were seeded onto the surface of HA-SLA-Ti and SLA-Ti respectively, the growth of MOOBs on the two kinds of samples were observed by scaning electron microscope.The proliferation index, alkaline phosphatase(AKP) activity, osteocalcin(BGP) content of MOOBs and mRNA relative expression level of osteopontin(opn) were examined and compared between two groups.Results: MOOBs grew well on the surface of HA-SLA-Ti; the proliferation index, AKP activity and BGP content of MOOBs were higher in HA-SLA-Ti group than those in SLA-Ti group(P<0.05); the relative expression level of opn mRNA in HA-SLA-Ti group was 3.25 times greater than that in SLA-Ti group.Conclusions:The HA coating formed by IBAD method on the SLA surface of titanium dental implants can improved the ability of proliferation and the expression of osteoblastic phenotype of MOOBs.