| 小鼠单纯疱疹病毒Ⅰ型脑内潜伏感染的实验研究 |
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| 引用本文： | 李平,谢鹏,王运良,李亚军,李勇,牟君.小鼠单纯疱疹病毒Ⅰ型脑内潜伏感染的实验研究[J].中国临床神经科学,2005,13(1):17-19. |
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| 作者姓名： | 李平 谢鹏 王运良 李亚军 李勇 牟君 |
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| 作者单位： | 重庆医科大学附属第一医院神经内科,400016 |
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| 摘 要： | 目的:探讨单纯疱疹病毒Ⅰ型(HSV-Ⅰ)F株初次感染后在脑内是否形成潜伏感染。方法:用角膜划痕法给Balb/c小鼠接种单纯疱疹病毒Ⅰ型F株,6周后用免疫组化法检测脑组织不同部位及三叉神经节HSV-1抗原的表达,聚合酶链反应(PCR)方法分别检测小鼠脑的颞叶、脑干、小脑及三叉神经节疱疹病毒DNA片段。结果:病毒接种6周后,小鼠三叉神经节、脑组织的不同部位未检测到HSV-1抗原的表达;三叉神经节、脑的颞叶、脑干和小脑均检测到了疱疹病毒DNA片段。结论:单纯疱疹病毒除了可以在三叉神经节建立潜伏感染外,脑组织也是其建立潜伏感染的部位之一。
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| 关 键 词： | 单纯疱疹病毒Ⅰ型 潜伏感染 脑组织 三叉神经节 |
| 文章编号： | 1008-0678(2005)01-0017-03 |
| 修稿时间： | 2004年9月15日 |
Experimental Study on the Latent Infection with HSV-1 in Brain in Mice |
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| LI Ping,XIE Peng,WANG Yun-Liang,et al.Experimental Study on the Latent Infection with HSV-1 in Brain in Mice[J].Chinese Journal of Clinical Neurosciences,2005,13(1):17-19. |
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| Authors: | LI Ping XIE Peng WANG Yun-Liang |
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| Institution: | LI Ping,XIE Peng,WANG Yun-Liang,et al Department of Neurology,the First Affiliated Hospital,Chongqing University of Medical Sciences,Chongqing 400016,China |
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| Abstract: | Aim:To study whether the latent infection of HSV-1 F strain can be established in the mice brain after the primary infection. Methods: Ocular abrasion method was used to inoculate the Balb/c mice. Immunohistochemistry and polymerase chain reaction(PCR) methods were used to detect the expression of HSV-1 antigen and DNA of HSV, respectively in trigeminal ganglion(TG) ,temporal lobe of brain,cerebelleum and brain stem. Results: Six weeks later the gene of HSV-1 was detected both in TG and in different parts of the brain, but the expression of antigen was negative. Conclusion: The latent infection of HSV can be established in brain as well as in TG of mice. |
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| Keywords: | HSV-1 latent infection brain trigeminal ganglion |
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