高糖通过激活人脐静脉内皮细胞ⅠκB-α/NF-κB途径诱导MCP-1的表达 |
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引用本文: | 戚忠林,;廖广菊,;张剑波,;倪娟,;王国平.高糖通过激活人脐静脉内皮细胞ⅠκB-α/NF-κB途径诱导MCP-1的表达[J].中华糖尿病杂志,2008(11):685-687. |
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作者姓名: | 戚忠林 ;廖广菊 ;张剑波 ;倪娟 ;王国平 |
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作者单位: | [1]湖北鄂州市中心医院,436000; [2]华中科技大学同济医学院附属同济医院病理研究所,436000; |
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基金项目: | 教育部新世纪优秀人才支持计划(NCET-04-0711) |
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摘 要: | 目的观察高糖诱导人脐静脉内皮细胞NF-κB的活性和单核细胞趋化蛋白1(MCP-1)的表达,探讨糖尿病并发动脉粥样硬化的发病机制。方法在培养的人脐静脉内皮细胞中加入不同浓度葡萄糖,检测内皮细胞中MCP-1mRNA、MCP-1蛋白的表达,NF-κB的活性及IκB-α的磷酸化水平。结果高糖明显地诱导了血管内皮细胞NF-κB的活性、MCP-1的表达及IκB-α的磷酸化;NF-κB活性抑制剂明显地降低了高糖所诱导的MCP-1的表达。结论高糖通过诱导血管内皮细胞IκB-α的磷酸化激活NF-κB,从而诱导了MCP-1的表达。提示在糖尿病并发动脉粥样硬化的发病过程中,高血糖通过激活血管内皮细胞IκB-α/NF-κB途径诱导MCP-1的表达,进而发挥了重要的作用。
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关 键 词: | 糖尿病 单核细胞趋化蛋白 核因子κB 内皮细胞 |
High glucose induces monocyte chemoattractant protein-1 expression via stimulating NF-κB activity mediated by ⅠκB-α phosphorylation in the cultured human umbilical vein endothelial cells |
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Institution: | QI Zhong-lin, LIAO Guang-ju, ZHANG Jian-bo, et al.( Department of Pathology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.) |
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Abstract: | Objective To understand the mechanism for diabetes-induced atherosclerosis by observing monocyte chemoattractant protein-1 (MCP-1) expression and NF-κB activity in cultured endothelial cells with high concentration of glucose. Methods Human umbilical vein endothelial cells were isolated and cultured in M199 medium. RT-PCR was conducted to detect MCP-1 mRNA expression and ELISA was used to detect MCP-1 protein expression. Electrophoretic mobility shift assay (EMSA) was performed for NF-κB activity and Western blot analysis was carried out to detect phosphorylation of ⅠκB-α. Results High concentration of glucose significantly stimulated NF-κB activity and MCP-1 expression in the cultured cells, and the NF-κB specific inhibitor peptide (NF-κB SN50) markedly blocked the high glucose-induced MCP-1 expression and phosphorylation of ⅠκB-α. Conclusions High concentration of glucose induces MCP-1 expression via stimulating NF-κB activity mediated by phosphorylation of ⅠκB-α in vascular endothelial cells. |
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Keywords: | Diabetes Monocyte chemoattractant protein-1 NF-κB Endothelial cells |
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