| 响应面分析法优选南方红豆杉中多糖的提取工艺 |
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| 引用本文： | 孔繁晟,李日许,蔡焕哲,陈泽丹,洪锐涛.响应面分析法优选南方红豆杉中多糖的提取工艺[J].中国药房,2014(7):614-617. |
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| 作者姓名： | 孔繁晟 李日许 蔡焕哲 陈泽丹 洪锐涛 |
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| 作者单位： | 广东药学院药科学院,广州510006 |
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| 基金项目： | 广东省中医药局建设中医药强省立项资助科研课题（No.20121i59） |
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| 摘 要： | 目的:优选南方红豆杉中多糖的提取工艺。方法:以多糖提取率为评价指标,以pH、提取时间、提取温度、液料比为考察因素,采用单因素试验与响应面分析相结合的方法,优选南方红豆杉中多糖的提取工艺。结果:最佳提取工艺为设定pH为5,提取时间为20 min,提取温度为60℃,液料比为30∶1(V/V);在该工艺条件下,多糖提取率为0.68%。结论:所选工艺合理、可行,可用于南方红豆杉中多糖的提取。
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| 关 键 词： | 南方红豆杉 多糖 超声提取 响应面分析法 |
Optimization of the Extraction Technology of Polysaccharides from Taxus chinensis by Response Surface Analysis |
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| KONG Fan-sheng,LI Ri-xu,CAI Huan-zhe,CHEN Ze-dan,HONG Rui-tao.Optimization of the Extraction Technology of Polysaccharides from Taxus chinensis by Response Surface Analysis[J].China Pharmacy,2014(7):614-617. |
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| Authors: | KONG Fan-sheng LI Ri-xu CAI Huan-zhe CHEN Ze-dan HONG Rui-tao |
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| Institution: | (College of Pharmaceutical Science, Guangdong Pharmaceutical University, Guangzhou 510006, China) |
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| Abstract: | OBJECTIVE： To optimize the extraction technology of polysaccharide from Taxus chinensis. METHODS： The extraction technology of polysaccharide from T. chinensis was optimized by single factor test and response surface analysis with the extraction rate of polysaccharide as index using pH value, extraction time, extraction temperature and ratio of drug to liquid as factors. RESULTS： The optimal extraction conditions were pH value of 5, extraction for 20 min, at 60℃, and ratio of drug to liquid 30：1 （V/V）. The extraction rate of polysaccharide was 0.68% under the condition. CONCLUSIONS： The optimized technology is reasonable and feasible, and can be used for the extraction of polysaccharide from T. chinensis. |
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| Keywords: | Taxus chinensis Polysaccharide Ultrasonic extraction Response surface analysis |
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