Modulation of neural noradrenaline and ATP release by angiotensin II and prostaglandin E2 in guinea-pig vas deferens |
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Authors: | Bernd Driessen Klaus Starke |
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Institution: | (1) Pharmakologisches Institut, Albert-Ludwigs-Universität Freiburg, Hermann-Herder-Strasse 5, D-79104 Freiburg i. Br., Germany |
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Abstract: | Effects of angiotensin II and prostaglandin E2 on contractions, release of noradrenaline and release of ATP elicited by electrical stimulation (210 pulses, 7 Hz) were studied in the isolated vas deferens of the guinea pig. Release of noradrenaline was assessed as overflow of tritium after preincubation with 3H]-noradrenaline. ATP was measured by means of the luciferin-luciferase technique. In some experiments postsynaptic a
1-adrenoceptors and P2X-purinoceptors were blocked by prazosin and suramin, respectively, to isolate the neural fraction of the overflow of ATP.Electrical stimulation elicited an overflow of tritium and ATP and, in the absence of prazosin and suramin, contraction. In the absence of prazosin and suramin, angiotensin II (1–100 nM) enhanced contractions as well as the evoked overflow of tritium and ATP. All parameters were increased by about the same percentage for a given concentration of angiotensin 11. The effect of prostaglandin E2 (1–100 nM) was complex. Contractions were mainly enhanced, the evoked overflow of tritium was reduced, whereas the evoked overflow of ATP was predominantly increased. No or almost no contraction remained in the presence of prazosin and suramin, and the evoked overflow of ATP was decreased to about 16%. Angiotensin II (1–100 nM) again enhanced the evoked overflow of tritium and ATP. Both were increased by about the same percentage for a given concentration of angiotensin II and also were increased by about the same percentage as obtained in the absence of prazosin and suramin. Prostaglandin E2 (1–100 nM) decreased the evoked overflow of tritium and ATP in the presence of prazosin and suramin, both by about the same percentage at a given prostaglandin E2 concentration.It is concluded that neural release of ATP, like the release of noradrenaline, is presynaptically facilitated by angiotensin II and depressed by prostaglandin E2. In the case of angiotensin II, increases in neural and postsynaptic ATP release contribute to the increase in ATP over flow observed in the absence of prazosin and suramin. In the case of prostaglandin E2, an increase in postsynaptic ATP release can override the reduction in neural ATP release and give rise to an increase in ATP overflow in the absence of prazosin and suramin. No evidence for a differential modulation of neural noradrenaline versus ATP release was found.
Correspondence to: B. Driessen at the above address |
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Keywords: | Guinea-pig vas deferens Noradrenaline release ATP release Cotransmission Suramin Prazosin Angiotensin II Prostaglandin E2 1 |
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