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Thermoresponsive Substrates used for the Expansion of Human Mesenchymal Stem Cells and the Preservation of Immunophenotype |
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| Authors: | Maria E. Nash Xingliang Fan William M. Carroll Alexander V. Gorelov Frank P. Barry Georgina Shaw Yury A. Rochev |
| Affiliation: | 1. Department of Applied Macromolecular Chemistry, Instituto de Ciencia y Tecnología de Polímeros, ICTP-CSIC, Juan de la Cierva 3, 28006, Madrid, Spain 2. School of Chemistry, National University of Ireland, Galway, Ireland 3. National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland 4. School of Chemistry and Chemical Biology, University College Dublin, Dublin, Ireland 5. Regenerative Medicine Institute, National University of Ireland Galway, Galway, Ireland |
| Abstract: | The facile regeneration of undifferentiated human mesenchymal stem cells (hMSCs) from thermoresponsive surfaces facilitates the collection of stem cells avoiding the use of animal derived cell detachment agents commonly used in cell culture. This communication proposes a procedure to fabricate coatings from commercially available pNIPAm which is both affordable and a significant simplification on alternative approaches used elsewhere. Solvent casting was used to produce films in the micrometer range and successful cell adhesion and proliferation was highly dependent on the thickness of the coating produced with 1 μm thick coatings supporting cells to confluence. 3T3 cell sheets and hMSCs were successfully detached from the cast coatings upon temperature reduction. Furthermore, results indicate that the hMSCs remained undifferentiated as the surface receptor profile of hMSCs was not altered when cells were detached in this manner. |
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