| 板蓝根乙酸乙酯提取物HPLC指纹图谱及体外抑菌活性的相关性分析 |
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| 引用本文： | 孔维军,赵艳玲,山丽梅,肖小河,郭伟英.板蓝根乙酸乙酯提取物HPLC指纹图谱及体外抑菌活性的相关性分析[J].中国药学,2007,16(4):282-287. |
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| 作者姓名： | 孔维军 赵艳玲 山丽梅 肖小河 郭伟英 |
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| 作者单位： | [1]中国人民解放军第302医院全军中药研究所,北京100039 [2]辽宁医学院药学院,辽宁锦州121001 |
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| 基金项目： | State Administration of Traditional Chinese Medicine （Grant No. 04-05ZP70） and Military the Tenth Five Foundation （Grant No. 06MA360）. |
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| 摘 要： | 目的考察板蓝根乙酸乙酯提取物的HPLC指纹图谱及体外抑菌活性的相关性。方法HPLC法建立十批不同来源板蓝根的乙酸乙酯提取物的指纹图谱采用微量量热法记录大肠杆菌在板蓝根乙酸乙酯提取物作用下生长代谢的热谱图。运用系统聚类分析法考察不同来源板蓝根之间的差异，结合体外抑菌活性进行相关分析。结果不同来源板蓝根乙酸乙酯提取物的HPLC指纹图谱与体外抑菌活性之间存在很好的相关性。结论采用HPLC指纹图谱和体外抑菌活性相关性分析的方法，可以发现板蓝根乙酸乙酯部位抑菌活性有效成分。
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| 关 键 词： | 板蓝根 HPLC指纹图谱 抑菌活性 相关分析 |
| 文章编号： | 1003-1057（2007）4-282-06 |
| 收稿时间： | 2007-05-15 |
| 修稿时间： | 2007年5月15日 |
Correlation analysis between the HPLC fingerprints and in vitro antibacterial activity of ethyl acetate extracts from Radix isatidis |
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| Wei-Jun Kong,Yan-Ling Zhao,Li-Mei Shan,Xiao-He Xiao,Wei-Ying Guo.Correlation analysis between the HPLC fingerprints and in vitro antibacterial activity of ethyl acetate extracts from Radix isatidis[J].Journal of Chinese Pharmaceutical Sciences,2007,16(4):282-287. |
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| Authors: | Wei-Jun Kong Yan-Ling Zhao Li-Mei Shan Xiao-He Xiao Wei-Ying Guo |
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| Institution: | Wei-Jun Kong, Yan-Ling Zhao, Li-Mei Shan, Xiao-He Xiao, Wei-Ying Guo(1. PLA Institute of Chinese Materia Medica, 302 Hospital of People＇s Liberation Army, Beijing 100039, China; 2. Pharmacy College, L iaoning Medical University, Liaoning Jinzhou 121001, China) |
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| Abstract: | Aim To study the correlation between the HPLC fingerprints and in vitro antibacterial activities of EtOAc extracts of Radix isatidis from various sources. Methods Ten batches of Radix isatidis EtOAc extracts were analyzed with HPLC and the fingerprints were established. The influence of EtOAc extracts on the thermogenic curve of growth of Escherchia coli was obtained by microcalorimetry. The chemical differences of EtOAc extracts of Radix isatidis from various sources in the HPLC fingerprints were probed with hierarchical clustering analysis and similarity analysis. The correlation between the HPLC fingerprints and in vitro antibacterial activities was analyzed with multivafiant correlation analysis. Results Close correlation existed between the HPLC fingerprints and in vitro antibacterial activities of EtOAc extracts of Radix isatidis. Conlusion The combination of HPLC fingerprints and antibacterial activities can be used to discover principle components of Radix isatidis on bioactivity. |
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| Keywords: | Radix isatidis HPLC Fingerprint Antibacterial activity Corrdation analysis |
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