Monoclonal antibodies to a specific 54-kilodalton antigen of Nocardia spp. |
| |
| Authors: | P Boiron D Stynen G Belkacem A Goris F Provost |
| |
| Affiliation: | Unité de Mycologie, Institut Pasteur, Paris, France. |
| |
| Abstract: | Two monoclonal antibodies (MAbs) of the immunoglobulin G2A isotype, reacting with a Nocardia-specific 54-kDa antigen, were generated. As determined by Western blot (immunoblot), both MAbs reacted only with the 54-kDa band. As determined by indirect immunofluorescence or enzyme immunoassay with whole microorganisms, the MAbs did not react with Nocardia cells. One of the MAbs showed weak cross-reactivity with mycobacterial antigens, while the other showed no cross-reactivity. |
| |
| Keywords: | |
|
|
