ACE基因多态性与运动耐力的相关性分析

目的探讨血管紧张素转化酶(ACE)基因多态性与运动耐力的相关性。方法以多聚酶链反应(PCR)方法检测68例长跑运动员以及60例年龄、性别相匹配的健康对照者的ACE基因型,紫外线吸收法测定ACE活性,放射免疫法检测血浆血管紧张素Ⅱ(AngⅡ)浓度。结果运动员组II基因型频率为0.529,I等位基因频率为0.721,显著高于对照组(分别为0.333和0.575),差异有显著性(χ2=4.732、7.316,P<0.05)。运动员组DD型ACE活性和血浆AngⅡ浓度较II型显著升高,差异有显著性(F=3.93、5.15,q=2.557、5.501,P<0.05)。结论优秀运动员运动耐力可能与ACE基因I等位基因相关,ACE基因II型运动员可能有更好的训练敏感性。

ANGIOTENSIN-CONVERTING-ENZYME GENE POLYMORPHISM AND SPORTS ENDURANCE:A CORRELATION ANALYSIS

Objective To investigate the relationship between angiotensin-converting-enzyme(ACE) gene polymorphism and physical endurance. Methods Using polymerase chain reaction method,ACE genotype in 68 long-distance running athletes,and 68 healthy controls with age-sex matched was detected.ACE activity was ditermined by ultraviolet absorbtion method,and angiotensin Ⅱ(Ang Ⅱ) levels in plasma by radioimmunity. Results The frequency of genotype II in athlete group was 0.529,and that of allele I was 0.721,being significantly higher than that in the control,which were 0.333 and 0.575,respectively(χ2=4.732,7.316;P<0.05).The levels of DD genotype ACE activity and plasma Ang Ⅱ in athlets were higher than that in genotype II,the differences being significant(F=3.93,5.15;q=2.557,5.501;P<0.05). Conclusion Physical endurance of outstanding athletes may have an association with ACE gene I allele;and athletes with ACE gene II genotype may have a preferable training sensitivity.