PI-103增强顺铂对C13K细胞裸鼠移植瘤生长的抑制作用

目的通过评价PI-103联用顺铂对C13K细胞体外增殖、凋亡及裸鼠移植瘤生长的影响为卵巢癌的靶向治疗寻找新的药物。方法C13K细胞经PI-103和／或顺铂处理后，以MTr法检测细胞的增殖情况，以流式细胞仪检测细胞凋亡，Western印迹检测p-AKT和p-S6K1的表达情况。建立C13K细胞皮下裸鼠移植瘤模型，随机分成对照组、PI-103组、顺铂组、合并组4组，连续用药4周。观察肿瘤生长情况，计算抑瘤率。结果PI-103联合顺铂抑制C13K细胞增殖，且随时间延长抑制作用越显著，抑制效果优于单独用药。PI-103可增强顺铂在C13K细胞中引起的凋亡。经PI-103作用后的C13K细胞表达p-AKT和P-S6K1较对照组下降，PI-103联合顺铂明显抑制裸鼠C13K细胞皮下移植瘤的生长，较顺铂单药组有明显差异性。结论PI-103增强顺铂对C13K细胞裸鼠移植瘤生长的抑制作用。

PI-103 Enhances Cisplatin-Caused Growth Inhibition of C13K Cell Xenografts in Nude Mice

Objective To examine the effect of PI-103 in combination with cisplatin on the proliferation and apoptosis of CI3K ceils and growth of xenografts of nude mice. Methods The pro- liferation of C13K cells was assessed by MTT assay after exposure to PI-103, cisplatin or both. Apoptosis was evaluated by flow cytometry. Immunoblot analysis was performed to detect the ex- pression of phosphorylation of AKT and S6 kinase 1. C13K cells was subcutaneously transplanted into nude mice （n = 16 ）, which were randomly divided into control group, PI-103 group, cisplatin group and combined treatment group. After treatment for 4 consecutive weeks, the tumor inhibition rate was evaluated. Results PI-103 in combination with cisplatin inhibited the proliferation of C13K cells in a time-dependent manner. The inhibitory effect of combination therapy was superior to PI-103 or cisplatin treatment alone. PI-103 increased cisplatin-induced apoptosis in C13K cells. PI-103 in- hibited the expression of phosphorylation of AKT and S6 kinase 1 in CI3K cells. PI-103 plus cispla- tin significantly inhibited the growth of xenografts in nude mice and there was significant differencebetween combined treatment group and cisplatin group. Conclusion Pl-103 may enhance growth in- hibition of C13K cell xenografts by cisplatin in nude mice.