染色体外游离态的子宫颈癌HPV16分离株E1／E2区的基因 …

目的 观察子宫颈癌组织中呈染色体外质粒状态的ＨＰＶ１６分离株Ｅ１／Ｅ２基因序列。方法 Ｓｏｕｔｈｅｒｎ转印杂交检测癌组织中ＨＰＶ１６基因组的物理状态。对８例游离状态的ＨＰＶ１６分离株Ｅ１／Ｅ２区进行ＰＣＲ扩增，克隆后酶切分析和ＤＮＡ序列分析。结果 对２８例ＨＰＶ１６阳性子宫颈癌中病毒ＤＮＡ杂效结果显示８例呈染色体外质粒方式存在。ＰＣＲ克隆鉴定发现８例分离株都具有完整的Ｅ１／Ｅ２区域。此外４例分离株

Presenceof genetic rearrangements in E1/E2 regions of episomal HPV 16 isolates from cervicalcarcinomas

Objective Analysis of genetic situations of E1/E2 region of episomal HPV 16 isolates from cervical carcinomas. Methods Physical statues of HPV 16 DNAs from cervical carcinomas were analyzed by Southern blot hybridization. E1/E2 regions of episomal HPV 16 isolates were amplified by PCR, and the PCR products were cloned and sequenced. Results Eight isolates from totally 28 cases showed extrachromosomal HPV 16 DNA. PCR cloned sequence analysis revealed that all the eight isolates contained intact E1/E2 regions, whereas 4 isolates had different ratios of genetic rearrangements, including insertion of E1 repeat sequence and several deletions. Only one case showed the same G deletion at position 1138 as HPV 16 prototype. Conclusion DNA rearrangements were present in E1/E2 regions of episomal HPV 16 isolates from cervical cancers and might play a role in tumorigenesis. Different viral genotypes in an identical tumor tissue might indicate that the tumor occurring at the same position of a patient may derive from two or even more cell clones.