藏药柳茶挥发油对小鼠急性心肌缺血损伤的干预作用

目的 研究藏药柳茶挥发油对垂体后叶素所致小鼠急性心肌缺血损伤的干预作用。方法 采用垂体后叶素腹腔注射建立小鼠急性心肌缺血损伤模型，给予柳茶挥发油治疗低、中、高剂量组干预后，监测小鼠造模前后心电图Ⅱ导联J点变化，检测血清过氧化氢酶（CAT）、谷胱甘肽转移酶（GSH-Px）、超氧化物歧化酶（SOD）活性以及乳酸（LD）的水平；显微镜下观察心肌缺血损伤的病理学变化及测定心肌梗死面积，并采用RT-PCR法测定组织caspase-3 mRNA的表达差异。结果 与模型组比较，柳茶挥发油中、高剂量组及阳性对照组缺血所致心电图J点的上移均显著降低（P<0.05）；血清CAT、GSH-Px、SOD活力明显增加（P < 0.05或P < 0.01），LD含量明显降低（P<0.01），病理学研究发现心肌损害程度减轻，缺血损伤面积明显减少，且高剂量干预组小鼠心肌组织的caspase-3 mRNA表达明显下调（P<0.05）。结论 藏药柳茶挥发油对小鼠急性心肌缺血损伤具有一定的保护作用，且与抑制caspase-3 mRNA的表达有关。

Intervention Effect of Sibiraea angustata Volatile Oil on Acute Myocardial Ischemic Injury in Mice

OBJECTIVE To evaluate the intervention effect of Sibiraea angustata volatile oil on pituitrin-induced acute myocardial ischemic injury in mice. METHODS The acute myocardial ischemic injury model was made by injecting pituitrin in mice and treated with Sibiraea angustata volatile oil, the change of the J spot was measured by electrocardiogram, the biochemical indicatorsCatalase(CAT), Glutathione transferase(GSH-Px), superoxide dismutase(SOD) and lactic acid(LD)] in surum were measured and compared; The heart were fixed for pathological investigation to observe the degree of myocardial ischemic injury and myocardial infarct size by optical microscope, the activity and mRNA expression of caspase-3 was measured by RT-PCR. RESULTS Compared with the model group, the moving up of J spots in the treated groups was significantly inhibited(P<0.05), the activities of SOD, GSH-Px and CAT were increased(P < 0.05 or P < 0.01), while the content of LD was decreased(P<0.05); Moreover, the histopathological research indicated Sibiraea angustata volatile oil significantly diminished the areas of cardiac muscles injured by ischemia. Mechanisms studies indicated that the mRNA expression of caspase-3 was decreased significantly(P<0.05). CONCLUSION Sibiraea angustata volatile oil has protective effect on acute myocardial ischemic injury in mice and related to the caspase-3 downregulation.