大鼠臂丛前根撕脱延期再植回对运动神经元存活的作用

目的：建立大鼠臂丛前根撕脱延期再植回模型，研究脊髓前角运动神经元的存活与再生。方法：采用FB逆行示踪法结合NADPH—d组化法与中性红复染，计数阳性神经元．结果：前根撕脱延期3、7、14d再植回组，动物存活3周及6周，脊髓前角运动神经元的存活率分别为79％和75％、58％和51％、57％和50％；NOS阳性神经元的表达率分别为18％和13％、30％和8％、20％和7％。仅做前根撕脱组动物，脊髓前角运动神经元存活率为41％和29％，NOS阳性神经元的表达率为55％和58％。结论：前根撕脱延期再植回能促进脊髓前角运动神经元的存活与再生。

Survival and regeneration of motoneurons in adult rats by delayed reimplantation of ventral root following spinal root avulsion

Objective: To establish the animal model of delaying reimplantation of avulsing ventral root and investigate the survival and regeneration of motoneurons. Methods: The positive neurons were counted after adopting fast-blue retrogradation label technique, NADPH-diaphoase histochemistry and neutral red counterstaining. Results: After 3 and 6 weeks' living period, the survive rate of positive motoneuron at 3, 7, 14 days after operation were 79% and 75%, 58% and 51%, 57% and 50% respectively, about 18% and 13%, 30% and 8%, 20% and 7% of motoneuron expressed NOS. The motoneuron survive rate of the group of avulsion without reimplantation were 41% and 29%, while the positive rate of NOS were 55% and 58%. Conclusions: Delayed reimplantation of avulsion ventral root can enhance motoneuron survival and regeneration.