抗角蛋白16单克隆抗体对角质形成细胞增殖活性的影响

目的研究抗角蛋白单克隆抗体(mAb)对体外培养的角质形成细胞增殖活性与细胞周期的影响.方法以无血清培养基体外原代培养角质形成细胞.应用3H-TdR掺入DNA合成测定法,观察抗角蛋白16mAb(anti-K16mAb)对角质形成细胞代谢增殖的影响.用流式细胞仪分析anti-K16mAb作用后角质形成细胞的细胞周期变化.结果mAb作用后,角质形成细胞样品的cpm降低,并呈抗体剂量依赖方式.流式细胞仪分析表明,处于S期的细胞比例由27%上升至42.2%,同时处于G1期与G2期的细胞比例分别相应下降.结论anti-K16mAb可抑制体外培养的角质形成细胞的增殖活性,且将细胞阻滞于S期,无法进入G2期与M期分裂增殖.

Influence of anti-keratin16 monoclonal antibody on proliferation of keratinocyte

Aim To study the influence of anti keratin monoclonal antibody on proliferation and cell cycle of human keratinocytes cultured in vitro. Methods Human keratinocytes were primarily cultured in vitro with serum free medium. The influences of anti keratin 16 monoclonal antibody (anti K16 mAb) on the proliferation and cell cycle of cultured keratinocytes were studied by 3H TdR incorporation assay and cytometer analysis, respectively. Results Cpm value of samples of keratinocytes treated with anti K16 mAb was decreased in dose dependent manner. Results from flow cytometer analysis showed that proportion of cells in S phase was increased from 27% to 42.2%, and that proportions of cells in G1 and G2 phases was decreased correspondingly. Conclusion Anti K16 mAb inhibits the proliferation of keratinocytes cultured in vitro, preventing them in S phase from entering G2 and M phases.