清开灵注射液对小鼠全氟异丁烯吸入性急性肺水肿的治疗作用

目的探讨清开灵注射液对全氟异丁烯(PFIB)吸入性急性肺水肿的治疗作用。方法小鼠以全身暴露动态吸入PFIB方式染毒,剂量为130 mg/m3染毒5 m in。在小鼠PFIB染毒后1 h腹腔注射清开灵注射液,剂量分别为1.75、3.50、7.00、14.00 m l/kg。以染毒小鼠存活率、肺系数、支气管肺泡灌洗液(BALF)中蛋白含量及弹性蛋白酶活力、组织病理学检查等为观测指标,观察清开灵注射液抗PFIB吸入性急性肺水肿作用的量效关系。结果清开灵注射液可显著降低PFIB染毒小鼠的湿(干)肺体比、肺含水量及肺湿/干比,明显降低BALF中的蛋白含量,显著提高PFIB染毒小鼠的存活率,改善染毒小鼠的肺组织病变,且量效关系良好。结论清开灵注射液对PFIB所致肺水肿与炎性渗出具有肯定的抑制作用,并能有效对抗PFIB所致动物死亡。

The therapeutic effect of Qingkailing injection on perfluoroisobutylene-inhalation induced acute pulmonary edema in mice

Objective To investigate the therapeutic effect of Qingkailing injection on experimental acute pulmonary edema induced by perfluoroisobutylene(PFIB) inhalation.Methods Mice were intoxicated by flow-past whole-body exposure to PFIB at the concentration of 130 mg/m~3 for 5 minutes.One hour after the exposure,they were administrated Qingkailing injection intraperitoneally at the doses of 1.75,3.50,7.00 and 14.00 ml/kg respectively.Indexed by mice survival rate,lung coefficients,protein content and elastase activity in bronchoalveolar lavage fluid(BALF),as well as histopathologic alterations post PFIB exposure,the dose-response relationship of Qingkailing injection against acute pulmonary edema induced by PFIB inhalation was observed.Results Qingkailing injection could significantly decrease the wet(dry) lung-to-body weight ratio,water content in lung,lung wet-to-dry weight ratio as well as protein content in BALF in the PFIB-exposed mice in a well dose-depen-(dent) manner,which were well supported by the dramatically increased survival rate and ameliorated histopathologic alterations in the lung.Conclusion Qingkailing injection has the ascertained inhibitive effect on PFIB-induced pulmonary edema and inflammatory exudation and can effectively fight against PFIB-induced animal death.