| ZnT7及游离锌离子在小鼠室管膜和脉络丛细胞的分布 |
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| 引用本文： | 池志宏,柳玉霞,张莉,于丹,王占友.ZnT7及游离锌离子在小鼠室管膜和脉络丛细胞的分布[J].解剖科学进展,2007,13(1):59-61. |
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| 作者姓名： | 池志宏 柳玉霞 张莉 于丹 王占友 |
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| 作者单位： | 中国医科大学基础医学院组织胚胎学教研室,辽宁,沈阳,110001;锦州市古塔医院,辽宁,锦州,121001;中国医科大学基础医学院组织胚胎学教研室,辽宁,沈阳,110001;朝阳市卫生学校,辽宁,朝阳,122000;中国医科大学基础医学院组织胚胎学教研室,辽宁,沈阳,110001 |
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| 摘 要： | 目的 研究锌转运体7（ZnT7）和游离锌离子在小鼠脊髓室管膜和脉络丛上皮细胞中的分布.方法 应用ZnSe金属自显影技术（AMG）检测硒酸钠注射1.5h后小鼠脊髓室管膜细胞及脑室脉络丛上皮细胞的游离锌离子;应用免疫组织化学SABC法检测小鼠脊髓室管膜细胞及脑室脉络丛上皮细胞中ZnT7的表达.结果 光镜下观察AMG染色的切片,小鼠脊髓室管膜细胞及脑室脉络丛上皮细胞中均有游离锌离子的分布;免疫组织化学结果表明,脊髓室管膜细胞及脉络丛上皮细胞中均有ZnT7的表达,且与游离锌离子分布区域基本一致.结论 锌离子可能在脊髓室管膜细胞及脉络丛上皮细胞内发挥重要作用,脊髓室管膜细胞及脉络丛上皮细胞可能在脑脊液锌转运过程中发挥重要的作用.
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| 关 键 词： | 游离锌离子 金属自显影技术 锌转运体 室管膜 脉络丛 |
| 文章编号： | 1006-2947（2007）01-0059-03 |
| 收稿时间： | 2006-12-20 |
| 修稿时间： | 2006年12月20 |
Distribution of ZnT7 Protein and Free Zinc Ions in Mouse Ependyma and Choroid Epithelium |
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| CHI Zhi-hong,LIU Yu-xia,ZHANG Li,YU Dan,WANG Zhan-you.Distribution of ZnT7 Protein and Free Zinc Ions in Mouse Ependyma and Choroid Epithelium[J].Progress of Anatomical Sciences,2007,13(1):59-61. |
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| Authors: | CHI Zhi-hong LIU Yu-xia ZHANG Li YU Dan WANG Zhan-you |
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| Institution: | CHI Zhi-hong LIU Yu-xia ZHANG Li YU Dan WANG Zhan-you ( 1. Department of Histology and Embryology, China Medical University, Liaoning Shenyang, 110(301 ; 2. Guta Hospital, Liaoning Jinzhou 121001 ; 3. Chaoyang Medical School, Liaoning Chaoyang 122000 China) |
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| Abstract: | Objective To investigate the localization of zinc transporter 7(ZnT7) protein and free zinc ions in ependyma cells in mouse spinal cord and choroid plexus cells in mouse brain.Methods Zinc selenide autometallography(AMG) was used to detect free zinc ions in mouse ependyma and choroid plexus 1.5 hours after sodium selenite treatment;SABC immunohistochemistry was used to detect the location of ZnT7 protein in ependyma and choroid epithelium.Results One and half hours after selenite intraperitoneal injection,almost all ependyma and choroid plexus cells showed AMG positive reactivity.ZnT7-immunoreactive cells were observed in almost all ependyma cells and choroid plexus cells,and the distribution of the immunoreactivity was similar to the AMG staining.Conclusion The abundant distribution of both ZnT7 protein and free zinc ions in mouse ependyma and choroid plexus cells suggests that zinc may play a important role in ependyma and choroid plexus. |
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| Keywords: | Zinc transporter free zinc ions autometallography ependyma choroid plexus |
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