125I 偶联VEGF反义寡核苷酸对人膀胱癌VEGF的抑制作用研究

 目的观察¹²⁵I偶联VEGF反义寡核苷酸（¹²⁵I-ASODN）对膀胱癌细胞VEGF表达的抑制情况.方法采用免疫组化法观察¹²⁵I-ASODN对膀胱癌细胞VEGF表达的影响,并观察条件培养液对内皮细胞生长的抑制作用.结果免疫组化结果显示对照组VEGF阳性细胞数为平均89.1/100个细胞, ¹²⁵I-Na处理组为90.3/100个细胞,ASODN组为10.3/100个细胞, ¹²⁵I-ASODN组为4.3/100个细胞, ¹²⁵I-ASODN组和ASODN组与对照组及¹²⁵I-Na组比较,差异有显著性意义（P〈0.05）;对照组、¹²⁵I-Na处理组、ASODN组、¹²⁵I-ASODN组D值分别为0.398±0.031,0.387±0.010,0.298±0.013和0.148±0.011, ¹²⁵I-ASODN组和ASODN组D值与对照组及¹²⁵I-Na组比较,差异有非常显著性（P〈0.01）.结论 ¹²⁵I-ASODN 能够明显抑制膀胱癌细胞的VEGF表达,可作为临床抗血管生成治疗膀胱癌的一种有效方法.

Inhibiting Effect of 125I-coupled Vascular Endothel ial Growth Factor ( VEGF) Antisense Oligonucleotides on Expression of VEGF in Bladder Cancer Cells

Objective 　To study the inhibition effect of VEGF expression in human bladder carcinoma cells by ¹²⁵I-coupled antisense oligonucleotides ( ¹²⁵I-ASODN) . Methods 　VEGF expression in human bladder cancer cells was detected with immunohistochemistry. Endothelial cell growth was assessed after adding conditional medium. Results 　By immunohistochemistry , the positive cells were 89. 1/ 100 cells in control group , 90. 3/ 100 cells in ¹²⁵I-Na group , 10. 3 in ASODN group and 4. 3 in 125 I2ASODN group. The D value of each group was 0. 398 ± 0. 031 ,0. 387 ±0. 010 ,0. 298 ±0. 013 and 0. 148 ±0. 011. Comparing ¹²⁵I-ASODN group and ASODN group with cont rol and ¹²⁵I-Na group , difference was significant ( P <0. 05) . Conclusion 　¹²⁵I-ASODN can effectively suppress VEGF expression of human bladder cancer cells. It has good prospect in canti-angiogenesis of bladder carcinoma.