HPLC法测定毛冬青胶囊中具栖冬青苷和毛冬青酸的含量

目的：建立HPLC法同时测定毛冬青胶囊中具栖冬青苷和毛冬青酸含量的方法,并对3个批次毛冬青胶囊中上述两种化合物进行定量分析。方法：Zorbax SB-C18（4.6 mm×150mm,5μm）色谱柱,流动相为甲醇-0.2%磷酸水溶液,梯度洗脱,检测波长215 nm,柱温40℃,流速1.0 mL·min-1,进样量20μL。结果：具栖冬青苷和毛冬青酸的线性范围分别为1.22-521.1μg·mL-1（r=0.9995）、1.23-289.6μg·mL-1（r=0.9991）,加样回收率分别为99.24%（RSD=0.68%）、99.53%（RSD=0.85%）。结论：该方法简便、准确、重复性好,可为毛冬青胶囊提供质量控制依据。

Determination of Pedunculoside and Ilexolic Acid in Maodongqing Capsules by HPLC

Objective： To establish an HPLC method for the determination of pedunculoside and ilexolic acid in Maodongqing capsules. Methods： The separation was carried out at 40 ℃ on an Agilent Zorbax SB-C18（4.6 mm×150 mm, 5 μm） column with methanol and 0.2% phosphoric acid as a mobile phase in a gradient elution. The flow rate was 1.0 mL·min-1and the injection volume was 20 μL. The detection wavelength was set at 215 nm. Results： Pedunculoside showed a good linear relationship at the range of1.22~521.1 μg·mL-1（r=0.9995）, the average recovery was 99.24% with RSD 0.68%. Ilexolic acid showed a good linear relationship at the range of 1.23~289.6 μg·mL-1（r=0.9991）, the average recovery was 99.53%with RSD 0.85%. Conclusion： The method is convenient, accurate with good reproducibility and can be used for quality control of Maodongqing capsules.