二甲氨基乙醇和复方氨基酸溶液对衰老 大鼠皮肤胶原代谢和自由基的影响

目的 探讨真皮内注射二甲氨基乙醇（DMAE）和复方氨基酸溶液（AA）对皮肤胶原代谢和自由基的影响.方法 80只大鼠随机分成衰老治疗组（60只）、衰老对照组（10只）和正常对照组（10只）.皮下注射半乳糖制备衰老模型.衰老治疗组从第18天开始,在两侧臀部真皮内分别注射0.2％ DMAE＋AA、0.1％ DMAE＋AA、0.2％ DMAE、0.1％ DMAE、AA、生理盐水（NS）,每周1次,连续4周.观察各组皮肤苏木精-伊红染色法（HE）切片组织学变化并测定皮肤中含水量、超氧化物歧化酶（SOD）活力、羟脯氨酸（HYP）和丙二醛（MDA）含量.结果 0.2％DMAE＋ AA组、0.1％ DMAE＋AA组表皮、真皮厚度和胶原纤维面积均高于衰老对照组,差异有统计学意义（P＜0.05）.0.2％ DMAE＋AA组、0.1％ DMAE＋ AA组与其他各衰老组相比,HYP含量增加、SOD活力升高、MDA含量降低,其组间差异具有统计学意义（P＜0.05）.正常对照组、0.2％ DMAE＋AA组、0.1％DMAE＋ AA组之间HYP含量差异无统计学意义（P＞0.05）.各组大鼠皮肤含水量差异无统计学意义（P＜0.05）.结论 真皮内注射0.2％ DMAE＋AA、0.1％ DMAE＋AA能显著增加表皮、真皮厚度和胶原纤维面积及HYP含量,提高SOD活力、降低MDA含量,表明其有清除皮肤自由基、提高抗氧化能力和促进皮肤胶原代谢的作用,从而延缓皮肤衰老.

Effect of dimethylaminoethanol and compound amino acid solution on collagen metabolism and free radicals in D-galactose induced aging rat skin

Objective To study the mechanism of anti-aging effect of dimethylaminoethanol （DMAE） and compound amino acid （AA） injection by mesotheray in D-galactose-induced skin aging rat.Methods Eighty rats were randomly divided into aging treatment group （60 cases）,aging control group （10 cases） and normal control group （10 cases）.The skin aging models were established by subcutaneous infectim of D-galactose.From the 18th day,the aging treatment group were injected intradermally in the rats＇ both sides hip skin with 0.2% DMAE＋ AA,0.1% DMAE＋AA,0.2%DMAE,0.1% DMAE,AA,and saline,once a week.After four weeks,HE,water content,superoxide dismutase （SOD） activity,hydroxyproline （HYP） and malondialdehyde （MDA） content were measured.Results Compared with the aging control group,the epidermal and the dermal thickness and the collagen area of rats skin＇ increased significantly in 0.2 % DMAE＋ AA and 0.1% DMAE＋ AA treatment groups （P〈0.05）.0.2% DMAE＋AA and 0.1 % DMAE＋AA treatment groups also had higher SOD activity,HYP content and lower MDA content than other groups （P〈0.05）,but no difference was noted among normal control group,0.2% DMAE＋AA and 0.1% DMAE＋AA group （P〉0.05）.There were no differences in water content among groups （P〉0.05）.Conclusions Intradermal injection with 0.1% DMAE＋ AA and 0.2 % DMAE＋AA in aging rats may increase the epidermal and the dermal thickness and the collagen of rats skin＇ improve SOD activity,HYP content and decreased MDA content,indicating that it has ability to clear skin free radicals,enhance antioxidant capacity and skin collagen metabolism,and thus prevent skin aging.