食管癌患者淋巴结转移灶DNA含量测定在预后判断中的意义

为了探讨食管癌患者癌周淋巴结转移(LNM)灶细胞DNA含量及其相关指标测定的预后意义,本文采用流式细胞术对64例食管癌癌周LNM灶细胞DNA含量进行检测,并对癌周LNM灶细胞的细胞生物学特性和患者预后的关系进行分析.食管癌癌周LNM灶细胞的DI值、H-检出率、SPF和PI均显著高于相应的对照组淋巴结,Apo却显著低于对照组;癌周LNM灶DNA二倍体者、倍体同质体者、SPF降低者、PI降低者、Apo升高者的术后生存期和5年生存率均显著高于DNA异倍体者、倍体异质体者、SPF升高者、PI升高者和Apo降低者.随癌周淋巴结转移阳性率的升高,患者生存期和5年生存率均逐渐降低,部分呈显著负相关关系. 结论:食管癌癌周LNM灶细胞DNA倍体变化是恶性肿瘤的重要生物学特性之一,其生物学特性和患者预后关系十分密切.因此,癌周LNM灶细胞的生物学参数可作为判断食管癌患者预后的重要参考及量化指标.

Clinical Significance of DNA Content of Lymph Node Metastasis in Patients with Esophageal Carcinoma

In order to study the clinical significance of the measurement on DNA content of lymph node metastasis in patients with esophageal carcinoma,the DNA content of tumor nests and para-cancer metastasis lymph nodes in 64 patients with esophageal carcinoma was measured by flow cytometry,as well as the correlation between biological characteristics of para-cancer metastasis lymph nodes and the prognosis of patients were analyzed.The results showed that the DI value,positive rates of H,SPF and PI in tumor nests and para-cancer metastasis lymph nodes in patients with esophageal carcinoma were respectively higher than those in control group,while Apo of them was significant lower than those in control group.The 5-year survival rate in patients with D,homogeneity of DNA content,SPF decreased,PI decrease and Apo increased were significantly higher than those in patients with H,heterogeneity of DNA content,SPF increased,PI increased and Apo decreased.The survival period and 5-year survival rate decreased gradually following the increase of para-cancer lymph node metastasis rate.They had significantly negative correlations in some cases.The results indicated that the change of DNA ploidy in tumor nests and para-cancer metastasis lymph nodes was one of significant biological characteristics of malignant tumors,which was closely related to the prognosis of patients.Therefore,the parameters of DNA content in para-cancer metastasis lymph nodes in patients with esophageal carcinoma may be regarded as a significant biological and quantitative reference for judging prognosis of the patients.