微流控芯片在肿瘤仿生模型构建中的应用

微流控芯片是一种在微米尺度下对流体进行精确操控的新技术,已被证明是对哺乳动物细胞及其微环境进行操控的理想平台.本文拟围绕肿瘤转移过程中的一些关键环节,对微流控芯片在肿瘤仿生模型构建中的应用作一综述.目前,基于微流控芯片技术构建的肿瘤仿生模型主要包括:肿瘤原发灶模型、肿瘤细胞诱导血管新生模型、肿瘤细胞内渗模型、肿瘤细胞外渗模型、肿瘤多器官转移模型等.相对于传统的体外研究方法,这些仿生模型很大程度上再现了体内的肿瘤微环境,逐渐成为肿瘤研究极重要的平台.

Biomimetic tumor models based on microfluidic chips

Microfluidic chips, a new technology for precisely manipulating fluids at the micron scale, have proven to be ideal platforms for manipulating mammalian cells and their microenvironments. This article aims to review the application of microfluidic chips in the construction of tumor biomimetic models around some key parts of tumor metastasis. Currently, tumor biomimetic models based on microfluidic chip technology mainly include tumor primary tumor model, tumor cell induced angiogenesis model, tumor cell infiltration model, tumor cell extravasation model and tumor multi-organ metastasis model. Compared with the traditional in vitro research methods, these bionic models reproduce the in vivo tumor microenvironment to a large extent and gradually become an important platform for tumor research.