通窍散瘀滴鼻液与微乳刺激性及其中药效成分葛根素大鼠在体鼻循环吸收比较研究

目的]本课题拟研究通窍散瘀滴鼻液和通窍散瘀微乳的刺激性,并比较通窍散瘀滴鼻液和通窍散瘀微乳的大鼠鼻腔吸收速率。方法]该研究采用蟾蜍上颚纤毛实验比较通窍散瘀滴鼻液和通窍散瘀微乳的刺激性。该研究以体积校正法,采用大鼠在体鼻循环吸收比较研究通窍散瘀滴鼻液与通窍散瘀微乳中有效成分葛根素大鼠鼻腔吸收特性。结果]结果表明,通窍散瘀滴鼻液的刺激性大于通窍散瘀微乳。通窍散瘀滴鼻液中葛根素吸收速率常数为(3.658±0.25)×10~(-3)/min,通窍散瘀微乳中葛根素吸收速率常数为(2.732±0.29)×10~(-3)/min。通窍散瘀微乳和通窍散瘀滴鼻液中葛根素的大鼠鼻腔吸收符合一级速率过程,为被动吸收。结论]通窍散瘀滴鼻液中葛根素的鼻腔吸收较通窍散瘀微乳快,但是刺激性大,为通窍散瘀鼻用制剂的开发提供了一定的依据。

Comparative study on the absorption of puerarin in the nasal circulation of Tongqiao stasis nasal drops and Tongqiao microemulsion

Objective] This study intends to study the irritation of Tongqiao nasal drops and Tongqiao microemulsion, and compare Tongqiao nasal drops and blood stasis Tongqiao microemulsion nasal absorption rate in rats.Methods] This study compared irritating Tongqiao nasal drops and Tongqiao microemulsion by using toad palate cilia experiment. Study on nasal absorption characteristics of active component of puerarin in Tongqiao stasis nasal drops and Tongqiao microemulsion in rats by volume correction method in rats with nasal circulation.Results] The results showed that irritation of Tongqiao nasal drops than Tongqiao microemulsion. The results showed that the rate constant of puerarin in the Tongqiao stasis nasal drops was (3.658±0.25)×10^-3/min, and the rate constant of puerarin in the microemulsion was (2.732±0.29)×10^-3/min. The nasal absorption of puerarin in Tongqiao nasal drops and Tongqiao microemulsion was consistent with the first-order rate process, which was the result of passive absorption.Conclusion] Compared with the Tongqiao microemulsion in nasal absorption of Puerarin, Tongqiao nasal drops is faster than microemulsion, but microemulsion is low irritation, as Tongqiao microemulsion nasal preparation development provides a certain basis for a new dosage.