Effects of Aphloia theiformis on key enzymes related to diabetes mellitus |
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Authors: | Marie Carene Nancy Picot |
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Institution: | Department of Health Sciences, Faculty of Science, University of Mauritius, Réduit, Mauritius |
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Abstract: | Context: Aphloia theiformis (Vahl.) Benn. (Flacourtiaceae) (AT) is traditionally used for the management of diabetes mellitus (DM), but there is no scientific data regarding activity against enzymes linked to this condition.Objective: To evaluate the kinetics of AT on key enzymes inhibition related to DM, and establish the antioxidant profile of AT.Materials and methods: Dried powdered AT leaves were used to prepare crude methanol extract (70% v/v) (CME). Kinetics of CME (5000 to 156.25?μg/mL) on α-amylase, α-glucosidase, and lipase inhibition were studied. CME was partitioned using solvents of increasing polarity and kinetics of enzyme inhibition of each fraction (1000–31.25?μg/mL) was evaluated. Potent fractions were combined to assess any synergistic effect. Total phenol, flavonoid, tannin, anthocyanin contents, and antioxidant capacity of AT was evaluated using standard spectrophotometric methods.Results: CME, ethyl acetate, and n-butanol fractions showed potent inhibitory activities against the enzymes with IC50 ranging from 22.94–939.97?μg/mL. Significant (p?0.05) reduction in IC50 (15.72 and 157.03?μg/mL against α-amylase and lipase, respectively) was observed when ethyl acetate and n-butanol fractions were combined; showing synergism. The extracts showed noncompetitive inhibition against α-amylase and α-glucosidase. Ethyl acetate, n-butanol fractions, and CME showed highest antioxidant capacities (0.44–1.41?μg GAE/mg sample), and phenol content (211.74-675.53?μg GAE/mg sample).Conclusion: This study supports the use of AT in the management of DM and provides the rationale for bioactivity guided isolation and characterization of compounds from the ethyl acetate and n-butanol fractions. |
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Keywords: | α-Amylase α-glucosidase pancreatic lipase Mauritius |
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