Antimutagenic and anticlastogenic effects of Turkish Black Tea on TA98 and TA100 strains of Salmonella typhimurium (in vitro) and mice (in vivo) |
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Authors: | Mohammad Charehsaz Hande Sipahi Ashok Kumar Giri |
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Affiliation: | Department of Toxicology-34755, Faculty of Pharmacy, Yeditepe University, Istanbul, Turkey |
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Abstract: | Context: Black tea has been reported to have significant antimutagenic and anticarcinogenic properties associated with its polyphenols theaflavins (TF) and thearubigins (TR). Similarly, Turkish black tea (TBT) also contains a considerable amount of TF and TR.Objective: This study investigated the mutagenic, antimutagenic and anticlastogenic properties of TBT.Materials and methods: The mutagenic and antimutagenic effects of TBT (10 to 40000?μg/plate) were investigated in vitro on Salmonella strains TA98 and TA100 with and without S9 fraction. Anticlastogenic effect was studied at concentrations of 300–1200?mg/kg TBT extract by chromosomal aberrations (CA) assay from bone marrow of mice.Results: The results of this study did not reveal any mutagenic properties of TBT. On the contrary, TBT extract exhibited antimutagenic activity at >1000?μg/plate concentrations in TA98 strain with and without S9 activation (40% inhibition with S9 and 27% without S9). In TA100 strain, the antimutagenic activity was observed at?>20,000?μg/plate TBT extracts without S9 activation (28% inhibition) and at >1000?μg/plate with S9 activation (59% inhibition). A significant decrease in the percentage of aberrant cells (12.33%?±?1.27) was observed in dimethylbenz(a)anthracene (DMBA) plus highest concentration (1200?mg/kg) of TBT extract-treated group when compared to only DMBA-treated group (17.00%?±?2.28).Discussion and conclusion: Results indicated that TBT can be considered as genotoxically safe, because it did not exert any mutagenic and clastogenic effects. As a result, TBT exhibited antimutagenic effects more apparently after metabolic activation in bacterial test system and had an anticlastogenic effect in mice. |
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Keywords: | Genotoxicity chromosomal aberration assay Ames test |
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