Amelioration of hyperglycaemia and modulation of antioxidant status by Alcea rosea seeds in alloxan-induced diabetic rats |
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Authors: | Parvaiz A. Dar Fasil Ali Ishfaq A. Sheikh |
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Affiliation: | 1. Clinical Biochemistry, University of Kashmir, Srinagar, India;2. King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia |
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Abstract: | Context: Alcea rosea L. (Malvaceae) has various medicinal uses including anticancer, anti-inflammatory and analgesic properties. However, there is no report on its antidiabetic activity.Objective: Alcea rosea seed extracts were evaluated for antihyperglycaemic and antioxidative potential in diabetic rats.Materials and methods: Single intra-peritoneal injection of alloxan (130?mg/kg b.w.) was used for induction of diabetes in Albino Wistar rats. Antihyperglycaemic and antioxidant activities of methanol and aqueous extracts of Alcea rosea seed (100 and 300?mg/kg b.w.), administered orally on daily basis for 15 days, were assessed in vivo for fasting blood glucose level and antioxidant status of liver and pancreas. Metformin was used as a positive control.Results: Aqueous and methanol extracts (300?mg/kg b.w.) decreased blood glucose level in diabetic rats by 24% and 46%, respectively. Administration of aqueous and methanol extracts at 300?mg/kg b.w. significantly (p?0.01) modulated the antioxidant status of liver in diabetic rats by increasing levels of GR (22.5?±?1.0, 24.4?±?1.02?μg GSSG utilized/min/mg of protein), GPx (20.7?±?1.2, 23.6?±?2.04?μg GSH utilized/min/mg of protein), SOD (36.1?±?1.7, 39.05?±?1.5 units/mg of protein) and CAT (1744.5?±?132.5, 1956.6?±?125.2?nmol H2O2 decomposed/min/mg of protein), respectively. Similar results were observed for pancreas.Discussion and conclusions: Antihyperglycaemic and antioxidative potentials of Alcea rosea seeds suggest its usefulness in management of diabetes and its complications. This is the first report on antidiabetic activity of this plant. |
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Keywords: | Hyperglycaemic oxidative stress glutathione antidiabetic phenolic compounds |
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