Phytochemical,antioxidant and protective effect of cactus cladodes extract against lithium-induced liver injury in rats |
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| Authors: | Anouar Ben Saad Brahmi Dalel Ilhem Rjeibi Amani Smida Sana Ncib Nacim Zouari |
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| Affiliation: | 1. Research Unit of Macromolecular Biochemistry and Genetics, Faculty of Sciences of Gafsa, University of Gafsa, Gafsa, Tunisia;2. Research Unit of Active Biomolecules Valorisation, High Institute of Applied Biology of Medenine, University of Gabes, Medenine, Tunisia;3. Laboratory of Research on Biologically Compatible Compounds, Faculty of Dental Medicine, University of Monastir, Gafsa, Tunisia;4. Common Services Unit, Faculty of Sciences Gafsa, University of Gafsa, Gafsa, Tunisia;5. High Institute of Applied Biology of Medenine, University of Gabes, Medenine, Tunisia |
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| Abstract: | Context: Opuntia ficus-indica (L.) Mill. (Castaceae) (cactus) is used in Tunisian medicine for the treatment of various diseases.Objective: This study determines phytochemical composition of cactus cladode extract (CCE). It also investigates antioxidant activity and hepatoprotective potential of CCE against lithium carbonate (Li2CO3)-induced liver injury in rats.Materials and methods: Twenty-four Wistar male rats were divided into four groups of six each: a control group given distilled water (0.5?mL/100?g b.w.; i.p.), a group injected with Li2CO3 (25?mg/kg b.w.; i.p.; corresponding to 30% of the LD50) twice daily for 30 days, a group receiving only CCE at 100?mg/kg of b.w. for 60 days and then injected with distilled water during the last 30 days of CCE treatment, and a group receiving CCE and then injected with Li2CO3 during the last 30 days of CCE treatment. The bioactive components containing the CCE were identified using chemical assays.Results: Treatment with Li2CO3 caused a significant change of some haematological parameters including red blood cells (RBC), white blood cells (WBC), haemoglobin content (Hb), haematocrit (Ht) and mean corpuscular volume (VCM) compared to the control group. Moreover, significant increases in the levels of glucose, cholesterol, triglycerides and of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) and lactate dehydrogenase (LDH) activities were observed in the blood of Li2CO3-treated rats. Furthermore, exposure to Li2CO3 significantly increased the LPO level and decreased superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx) activities in the hepatic tissues.Conclusion: CCE possesses a significant hepatoprotective effect. |
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| Keywords: | Alkali metal oxidative stress histopathology liver damage |
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