金纳多与茶多酚对缺氧诱导血管内皮细胞分泌内皮素的保护作用比较

目的观察缺氧对血管内皮细胞（VEC）分泌内皮素（ET）的影响及金纳多和茶多酚的保护作用。方法将VEC分为空白对照组、单纯缺氧组、缺氧＋金纳多组、缺氧＋茶多酚组。除空白对照组外，其他各组置于低压舱内进行缺氧暴露。用放免法测定各组缺氧前和缺氧后0．5、6、24h ET含量。结果（1）缺氧可促进VEC分泌ET增加（P〈0．01）。（2）同单纯缺氧组比较，在缺氧后0．5、6、24h时，金纳多组的ET含量明显低于缺氧组（P〈0．01）。（3）同单纯缺氧组比较，在缺氧后0．5h，茶多酚组的ET含量无显著性差异（P〉0．05）。在缺氧后6h和24h时，茶多酚组的ET含量明显低于缺氧组（P〈0．01）。（4）金钠多组与茶多酚组比较，金纳多组ET浓度在缺氧后0．5h较茶多酚组显著降低（P〈0．01）。在6h和24h两组比较无显著性差异（P〉0．05）。结论缺氧可显著增加VEC分泌ET；金纳多和茶多酚具有抑制缺氧促VEC分泌ET的作用。

Different Protective Effect Between Ginaton and Tea Polyphenol on Hypoxia-induced Endothelin Secretion from Vascular Endothelial Cells

Aim To explore the effects of ginaton and tee polyphenols(TPs) on vascular endothelial cells(VEC) Secretion of endothelin(ET) under hypoxia conditions.Methods VEC's were divided into 4 groups:control group,simple hypoxia group,hypoxia+Ginaton group and hypoxia+TPs group.The last three groups underwent hypoxia that was produced by the hypobaric chamber.The ET content before hypoxia and 0.5h,6h as well as 24h after hypoxia were measured with radioimmunoassay.Results(1)Simple hypoxia promoted the VEC's secreti...