| Survivin siRNA对肺癌A549细胞吉西他滨化疗敏感性的影响 |
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| 引用本文： | 武永存,吕喜英. Survivin siRNA对肺癌A549细胞吉西他滨化疗敏感性的影响[J]. 承德医学院学报, 2010, 27(1): 3-5. DOI: 10.3969/j.issn.1004-6879.2010.01.002 |
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| 作者姓名： | 武永存 吕喜英 |
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| 作者单位： | 承德医学院附属医院肿瘤放化疗科,河北承德,067000;承德医学院附属医院肿瘤放化疗科,河北承德,067000 |
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| 摘 要： | 目的：探讨SurvivinsiRNA对肺癌A549细胞吉西他滨化疗敏感性的影响。方法：肺癌A549细胞分为siRNA转染组、吉西他滨组、siRNA转染＋吉西他滨组和正常对照组；噻唑蓝比色法（MTT法）检测各组细胞增殖率的变化，流式细胞仪分析各组细胞的细胞周期和存活率。结果：SurvivinsiRNA＋吉西他滨组细胞的增殖率和存活率均明显低于siRNA转染组和吉西他滨组（P〈0．01）；且siRNA＋吉西他滨组与siRNA转染组、吉西他滨组比较，更高比率的A549细胞被阻滞于S期（P〈0．01）。结论：siRNA沉默Survivin表达，增加了肺癌A549细胞对吉西他滨的化疗敏感性。
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| 关 键 词： | 肺癌A549细胞 siRNA Survivin 吉西他滨 |
EFFECTS OF SURVIVIN SIRNA ON CHEMOSENSITIVITY OF LUNG CANCER CELLS A549 TO GEMCITABINE |
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| WU Yong-cun,LV Xi-ying. EFFECTS OF SURVIVIN SIRNA ON CHEMOSENSITIVITY OF LUNG CANCER CELLS A549 TO GEMCITABINE[J]. Journal of Chengde Medical College, 2010, 27(1): 3-5. DOI: 10.3969/j.issn.1004-6879.2010.01.002 |
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| Authors: | WU Yong-cun LV Xi-ying |
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| Affiliation: | Department of Chemoradiation;the Affiliated Hospital of Chengde Medical College;Hebei Chengde 067000;China |
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| Abstract: | Objective:To investigate the effects of Survivin siRNA on chemosensitivity of lung cancer cellsA549 to gemcitabine in vitro.Methods:Lung cancer cells A549 were divided into 4 groups:Survivin siRNA group,gemcitabine group,Survivin siRNA+gemcitabine group and normal control group.The effects of Survivin siRNA and gemcitabine on cell proliferation,cell cycle and cell survival were analyzed by MTT assay and flow cytometry respectively.Results:The proliferation rate and survival rate of cells in Survivin siRNA+g... |
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| Keywords: | Lung cancer cells A549 siRNA Survivin Gemcitabine |
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