醇取丹参液对几种口腔致病菌的体外抑菌作用

目的:研究95%醇取丹参溶液对几种口腔常见致病菌的体外抑菌作用。方法:采用杯碟法检测丹参提取液对牙龈卟啉菌、伴放线放线杆菌、变形链球菌、乳酸杆菌的抑菌活性及最小抑菌浓度,并检测pH值的改变对其抑菌作用的影响。采用SPSS10.0统计软件包进行t检验。结果:95%醇取丹参液对牙龈卟啉菌、伴放线放线杆菌、变形链球菌、乳酸杆菌均有抑菌作用,最小抑菌浓度分别为15.62、15.62、62.50和15.62mg/ml。pH值由3.5上升到7.0时,对伴放线放线杆菌、变形链球菌、乳酸杆菌的抑菌活性减弱(P<0.05),对牙龈卟啉菌的抑菌活性无影响(P>0.05)。结论:95%醇取丹参溶液对口腔常见致病菌有抑制作用。

An in vitro experiment on the antimicrobial effects of ethanol extract from salvia miltiorrhiza bunge on several oral pathogenic microbes

PURPOSE: To study the in vitro antimicrobial activity of ethanol extract of salvia miltiorrhiza bunge on several oral pathogenic microbes. METHODS: Antimicrobial activities of ethanol extract of salvia miltiorrhiza bunge for porphyromonas gingivalis ATCC 33277, A. actinomycetes comitans ATCC 24523, Streptococcus mutans, and Lactobacillus were determined using the cup-plate method.The minimum antibacterial concentrations of ethanol extract of salvia miltiorrhiza bunge were measured. SPSS10.0 software package was used for Student's t test. RESULTS: Ethanol extract of salvia miltiorrhiza bunge had antimicrobial activities on Porphyromonas gingivalis, A.actinomycetemcomitans,Streptococcus mutans, Lactobacillus, and the minimum inhibitory concentrations were 15.62 mg/ml,15.62 mg/ml,62.50 mg/ml and 15.62mg/ml; The pH of the solution was influential to its antimicrobial activity. CONCLUSION: Ethanol extract of salvia miltiorrhiza bunge has an antimicrobial activity on oral pathogenic microbes. Supported by Research Project of Science and Technology Bureau of Qingdao City (2001-038).