Binl—b蛋白及其mRNA在少弱精子症模型大鼠附睾中的表达

目的:验证Binl—b蛋白及其mRNA在少弱精子症模型大鼠附睾中的表达,探讨Binl—b在病理状态下附睾中表达的改变。方法:取雄性大鼠30只,随机分为两组,模型组给予雷公藤(10mg/ kg/d)灌胃,连用8W成少弱精子症模型;对照组给予1%羧甲基纤维钠混悬液灌胃。采用免疫组化S—P法和原位杂交方法检测Binl—b蛋白和Binl—bmRNA在两组大鼠附睾组织中的表达情况。结果:Binl—b蛋白表达于对照组、模型组大鼠的附睾管腔上皮、主细胞、基细胞,但在模型组中表达明显增强(P<0.01)。Binl—bmRNA表达于模型组、对照组附睾管腔上皮、主细胞、基细胞,但其在模型组中的表达明显增强(P<0.01)。结论:Binl—b蛋白及其mRNA在少弱精子症大鼠附睾内表达较正常大鼠明显增强,提示Binl—b可能是病理状态下雄性大鼠生殖系统的内源性防御分子之一。

The Expression of Binl-b Protein and mRNA in the Epididymis of Oligoasthenozoospermia Rat Models

Objective:To examine the expression of Binl-b protein and mRNA in the epididymis of oligoas- thenozoospermia rats,and probe into the changes of Binl-b expression in the epididymis under pathologic state.Methods:Sprague-Dwaley male rats (n=30) were randomly divided into 2 groups:the rats of mod- el group were intragastric administrated with triptolide (10mg/kg/d) for 8 weeks;and the rats of control group were intragastric administrated with 1% sodium carboxymethyl cellulose.Immunohistochemistry and in situ hybridization were used to examine the expression of Binl-b protein and mRNA in the epididymis of both groups.Results:In the model and control groups,the expression of Binl-b protein and mRNA were detected in the endepidermis,principal cells and basal cells of epididymis.And the level of Binl-b protein and mRNA expression were markedly increased in oligoasthenozoospermia model group (P<0.01).Conclu- sion:The expression of Binl-b protein and mRNA increase in the epididymis of oligoasthenozoospermia rats.It shows that Binl-b may be an endogenous defensive member of reproductive system of male rats un- der pathologic state.