金黄色葡萄球菌α毒素对肺水通道蛋白表达的影响

目的:观察金黄色葡萄球菌α毒素(α-toxin)对Balb/c小鼠肺微血管内皮细胞(LMEC)水通道蛋白1(AQP1)表达的影响。方法:将实验组小鼠15只腹腔注入α-toxin后根据6h、10h、24h取材时间,随机将它们分为α-toxin6h组、α-toxin10h组和α-toxin24h组。取左肺行病理HE染色和免疫组化染色,检测肺组织病理变化情况和AQP1表达的变化。结果:α-toxin各组肺泡间隔增宽、水肿,炎性细胞浸润,肺泡结构破坏,以α-toxin10h组最为明显。免疫组化α-toxin各组AQP1表达下降(P<0.05)。其中以α-toxin10h组最为明显。α-toxin6h组和α-toxin10h组及α-toxin10h组和α-toxin24h组均有显著性差异(P<0.05)。结论:AQP1表达于LMEC。金葡菌α毒素可致小鼠肺LMEC的AQP1表达下降,随着炎症的减轻,AQP1也随之表达增加,进而恢复正常。

Effect of S. Aureus α-toxin on pulmonary aquaporin-1 expression in mice

Objective:To observe the effect of S.aureus alpha toxin on pulmonary aquaporin-1(AQP1) expression in mice.Methods:Balb/c mice were divided into alpha toxin-6 h group,alpha toxin-10 h group and alpha toxin-24 h group according to the sampling time after injecting alpha toxin peritoneally.Immunohistochemistry stain for pulmonary AQP1 was done after execution.Results:Edema and inflammatory cell infiltration were shown in alveolar wall in all alpha toxin groups,especially obviously in alpha toxin-10 h group.AQP1 expression decreased in these groups,of which alpha toxin-10 h group was most obvious.Conclusion:AQP1 is mainly localized on lung microvascular endothelial cells.AQP1 expression decreased after mice are exposed to S.aureus alpha toxin,while down-regulated AQP1 return to normal level overtime.