达英-35治疗后PCOS患者卵泡液中睾酮及白血病抑制因子水平的变化

目的:通过检测卵泡液中白血病抑制因子(LIF)及睾酮(T)的变化,探讨达英-35改善卵泡发育的机理。方法:选择40例PCOS患者作为研究对象,随机分为用药组和不用药组各20例,选择19例无排卵障碍的患者作为对照组。用药前后分别在B超引导下抽取卵泡液进行白血病抑制因子及睾酮的检测。结果:①用药干预前LIF浓度,用药组为(19.6±3.6)ng/L,对照组为(34.9±3.6)ng/L,用药组与对照组比较明显降低(P<0.05)。T浓度用药组为(8.3±0.9)μg/L,对照组为(5.0±1.1)μg/L,用药组较对照组明显升高(P<0.05)。不用药组LIF浓度为(19.4±3.2)ng/L,T浓度为(8.2±1.0)μg/L,用药组与不用药组LIF及T浓度比较无统计学差异。②用药干预后LIF及T浓度变化:用药组与对照组LIF浓度分别为(27.9±6.0)ng/L、(35.3±3.7)ng/L,用药组仍低于对照组(P<0.05);T浓度用药组与对照组分别为(7.2±1.3)μg/L、(5.1±0.9)μg/L。不用药组LIF浓度为(19.6±3.1)ng/L,用药组高于不用药组(P<0.05);但T浓度两组无差异。用药组用药后LIF浓度较用药前升高(P<0.05);T浓度变化不明显。③用药干预后卵泡发育情况与LIF及T浓度变化幅度的关系:用药组卵泡成熟者7例,卵泡液LIF浓度增加幅度为(16.3±3.2)ng/L,而未成熟者增加幅度为(7.4±1.5)ng/L,两者有统计学差异(P<0.05);T浓度则在成熟卵泡中下降幅度大于未成熟者,两者有统计学差异(P<0.05)。结论:达英-35改善卵泡液发育可能与改变卵泡中白血病抑制因子及睾酮的水平有关。

Change of LIF and T in follicular fluid after treatment by the Dane35 on patients with PCOS

Objective:To study the mechanism of improvement in follicular development after using the Dane35 by detecting leukaemia inhibitory factor(LIF) and testosterone(T) in follicular fluid.Methods:19 patients with normal ovulation were taken as control group.40 patients with PCOS were taken as study group and divided into two subgroups,one subgroup was given the Dane35 three months.Follicular fluid were draw before and after treatment under B-scan in every women,LIF and T were detected in follicular fluid by ELIS...