何首乌含药血清对体外培养的人肝细胞活性的影响

目的：观察何首乌含药血清对体外培养的人肝细胞活性的影响,考察何首乌入血成分是否具有肝细胞毒作用。方法：采用血清药理学方法制备何首乌含药血清及空白对照组血清,作用于体外培养的人正常肝L02细胞和肝癌HepG2细胞,MTT法检测不同浓度血清对两种细胞活力的影响;倒置相差显微镜观察各浓度血清作用后两种细胞的形态变化;测定含药血清作用于两种细48 h后,细胞培养液转氨酶活性变化。结果：何首乌含药血清对人正常肝L02细胞和肝癌HepG2细胞的活力没有明显影响。两种细胞含药血清组与空白血清组相比,细胞形态都没有发生明显改变,细胞培养液转氨酶活性（ALT、AST）也没有显著差异（P〉0.05）。结论：何首乌含药血清对体外培养的两种人源肝细胞活性均没有明显影响。

Effects of Polygonum Multiflorum Extracts Serum on Cell Proliferation in Cultured Human Hepatocytes

Objective：This essay aims to prepare rat serum containing Polygonum multiflorum extracts（ PME） and to investigate whether the component into the blood can cause human liver cell damage. Methods： Normal human liver L02 cells and liver cancer HepG2 cells were treated by fetal bovine serum,serum from the rats in experimental group and control group. Cell growth inhibition of each group was determined by MTT method. The possible liver cell injury induced by the rat serum containing PME were clarified by cell morphological observation. Alanine aminotransferase（ALT） and aspartate aminotransferase（AST） activity in cells were determined by Reitman＇s. Results： PME-- containing medicated serum did not affect proliferation and transaminase activity of L02 cells and HepG2 cells（P〈0. 05）. Cell morphological observation showed no significant inhibition on both cells growth. Conclusion： PME-- containing medicated serum did not significantly affect the activity of the two human hepatocytes.